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Cyanobacterial cytotoxicity versus toxicity to brine shrimp Artemia salina
D. Hisem, P. Hrouzek, P. Tomek, J. Tomšíčková, E. Zapomělová, K. Skácelová, A. Lukešová, J. Kopecký
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- Artemia účinky léků MeSH
- bakteriální toxiny toxicita MeSH
- buněčné linie MeSH
- dlouhověkost účinky léků MeSH
- hodnocení rizik metody MeSH
- larva účinky léků růst a vývoj MeSH
- lymfocyty účinky léků metabolismus MeSH
- mikrocystiny toxicita MeSH
- mořské toxiny toxicita MeSH
- myši MeSH
- reprodukovatelnost výsledků MeSH
- sinice chemie metabolismus MeSH
- testy toxicity MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Heterocytous cyanobacteria from various habitats were screened for toxicity to brine shrimp Artemia salina and the murine lymphoblastic cell line Sp/2 in order to compare these two testing models for evaluation of risk posed by cyanobacteria to human health. Methanol extracts of biomass and cultivation media were tested for toxicity and selected extracts were fractionated to determine the active fraction. We found a significant toxic effect to A. salina and to Sp/2 cells in 5.2% and 31% of studied extracts, respectively. Only 8.6% of the tested strains were highly toxic to both A. salina and the Sp/2 cell line, and only two of the tested strains were toxic to A. salina and not to the murine cell line. Therefore, it is likely that the toxic effect of cyanobacterial secondary metabolites mostly targets basal metabolic pathways present in mammal cells and so is not manifested in A. salina. We conclude that it is insufficient to monitor cytotoxicity of cyanobacteria using only the brine shrimp bioassay as was usual in the past, since cytotoxicity is a more frequent feature in cyanobacteria in comparison with toxicity to A. salina. A. salina toxicity test should not be used when estimating the possible health risk for humans. We suggest that in vitro mammal cells be used for these purposes.
Citace poskytuje Crossref.org
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