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Cell death/proliferation and alterations in glial morphology contribute to changes in diffusivity in the rat hippocampus after hypoxia-ischemia
M. Anderova, I. Vorisek, H. Pivonkova, J. Benesova, L. Vargova, M. Cicanic, A. Chvatal, E. Sykova
Language English Country United States
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2010
PubMed Central
from 2010 to 1 year ago
Europe PubMed Central
from 2010 to 1 year ago
ProQuest Central
from 2000-08-01 to 2015-12-31
Open Access Digital Library
from 1996-01-01
Health & Medicine (ProQuest)
from 2000-08-01 to 2015-12-31
- MeSH
- Astrocytes pathology MeSH
- Cell Death MeSH
- Time Factors MeSH
- Diffusion MeSH
- Diffusion Magnetic Resonance Imaging MeSH
- Extracellular Space metabolism MeSH
- Gliosis etiology pathology MeSH
- CA1 Region, Hippocampal pathology physiopathology MeSH
- Hypoxia complications pathology physiopathology MeSH
- Immunohistochemistry MeSH
- Brain Ischemia complications pathology physiopathology MeSH
- Microscopy, Confocal MeSH
- Rats MeSH
- Neuroglia pathology MeSH
- Cell Count MeSH
- Rats, Wistar MeSH
- Cell Proliferation MeSH
- Reperfusion MeSH
- Body Water metabolism MeSH
- Imaging, Three-Dimensional MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
To understand the structural alterations that underlie early and late changes in hippocampal diffusivity after hypoxia/ischemia (H/I), the changes in apparent diffusion coefficient of water (ADC(W)) were studied in 8-week-old rats after H/I using diffusion-weighted magnetic resonance imaging (DW-MRI). In the hippocampal CA1 region, ADC(W) analyses were performed during 6 months of reperfusion and compared with alterations in cell number/cell-type composition, glial morphology, and extracellular space (ECS) diffusion parameters obtained by the real-time iontophoretic method. In the early phases of reperfusion (1 to 3 days) neuronal cell death, glial proliferation, and developing gliosis were accompanied by an ADC(W) decrease and tortuosity increase. Interestingly, ECS volume fraction was decreased only first day after H/I. In the late phases of reperfusion (starting 1 month after H/I), when the CA1 region consisted mainly of microglia, astrocytes, and NG2-glia with markedly altered morphology, ADC(W), ECS volume fraction and tortuosity were increased. Three-dimensional confocal morphometry revealed enlarged astrocytes and shrunken NG2-glia, and in both the contribution of cell soma/processes to total cell volume was markedly increased/decreased. In summary, the ADC(W) increase in the CA1 region underlain by altered cellular composition and glial morphology suggests that considerable changes in extracellular signal transmission might occur in the late phases of reperfusion after H/I.
References provided by Crossref.org
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- $a To understand the structural alterations that underlie early and late changes in hippocampal diffusivity after hypoxia/ischemia (H/I), the changes in apparent diffusion coefficient of water (ADC(W)) were studied in 8-week-old rats after H/I using diffusion-weighted magnetic resonance imaging (DW-MRI). In the hippocampal CA1 region, ADC(W) analyses were performed during 6 months of reperfusion and compared with alterations in cell number/cell-type composition, glial morphology, and extracellular space (ECS) diffusion parameters obtained by the real-time iontophoretic method. In the early phases of reperfusion (1 to 3 days) neuronal cell death, glial proliferation, and developing gliosis were accompanied by an ADC(W) decrease and tortuosity increase. Interestingly, ECS volume fraction was decreased only first day after H/I. In the late phases of reperfusion (starting 1 month after H/I), when the CA1 region consisted mainly of microglia, astrocytes, and NG2-glia with markedly altered morphology, ADC(W), ECS volume fraction and tortuosity were increased. Three-dimensional confocal morphometry revealed enlarged astrocytes and shrunken NG2-glia, and in both the contribution of cell soma/processes to total cell volume was markedly increased/decreased. In summary, the ADC(W) increase in the CA1 region underlain by altered cellular composition and glial morphology suggests that considerable changes in extracellular signal transmission might occur in the late phases of reperfusion after H/I.
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