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Cell death/proliferation and alterations in glial morphology contribute to changes in diffusivity in the rat hippocampus after hypoxia-ischemia
M. Anderova, I. Vorisek, H. Pivonkova, J. Benesova, L. Vargova, M. Cicanic, A. Chvatal, E. Sykova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 2010
PubMed Central
od 2010 do Před 1 rokem
Europe PubMed Central
od 2010 do Před 1 rokem
ProQuest Central
od 2000-08-01 do 2015-12-31
Open Access Digital Library
od 1996-01-01
Health & Medicine (ProQuest)
od 2000-08-01 do 2015-12-31
PubMed
20877389
DOI
10.1038/jcbfm.2010.168
Knihovny.cz E-zdroje
- MeSH
- astrocyty patologie MeSH
- buněčná smrt MeSH
- časové faktory MeSH
- difuze MeSH
- difuzní magnetická rezonance MeSH
- extracelulární prostor metabolismus MeSH
- glióza etiologie patologie MeSH
- hipokampální oblast CA1 patologie patofyziologie MeSH
- hypoxie komplikace patologie patofyziologie MeSH
- imunohistochemie MeSH
- ischemie mozku komplikace patologie patofyziologie MeSH
- konfokální mikroskopie MeSH
- krysa rodu rattus MeSH
- neuroglie patologie MeSH
- počet buněk MeSH
- potkani Wistar MeSH
- proliferace buněk MeSH
- reperfuze MeSH
- tělesná voda metabolismus MeSH
- zobrazování trojrozměrné MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
To understand the structural alterations that underlie early and late changes in hippocampal diffusivity after hypoxia/ischemia (H/I), the changes in apparent diffusion coefficient of water (ADC(W)) were studied in 8-week-old rats after H/I using diffusion-weighted magnetic resonance imaging (DW-MRI). In the hippocampal CA1 region, ADC(W) analyses were performed during 6 months of reperfusion and compared with alterations in cell number/cell-type composition, glial morphology, and extracellular space (ECS) diffusion parameters obtained by the real-time iontophoretic method. In the early phases of reperfusion (1 to 3 days) neuronal cell death, glial proliferation, and developing gliosis were accompanied by an ADC(W) decrease and tortuosity increase. Interestingly, ECS volume fraction was decreased only first day after H/I. In the late phases of reperfusion (starting 1 month after H/I), when the CA1 region consisted mainly of microglia, astrocytes, and NG2-glia with markedly altered morphology, ADC(W), ECS volume fraction and tortuosity were increased. Three-dimensional confocal morphometry revealed enlarged astrocytes and shrunken NG2-glia, and in both the contribution of cell soma/processes to total cell volume was markedly increased/decreased. In summary, the ADC(W) increase in the CA1 region underlain by altered cellular composition and glial morphology suggests that considerable changes in extracellular signal transmission might occur in the late phases of reperfusion after H/I.
Citace poskytuje Crossref.org
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