Detail
Article
Web resource
FT
Medvik - BMC
  • Something wrong with this record ?

Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen

A. Illichmanová, M. Janů, V. Sedláček, I. Kučera

. 2011 ; 56 (1) : 72-76.

Language English Country Czech Republic

Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12027706

A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV·](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV·](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV·](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12027706
003      
CZ-PrNML
005      
20151021093733.0
007      
ta
008      
120817s2011 xr d f 000 0eng||
009      
AR
024    7_
$a 10.1007/s12223-011-0004-6 $2 doi
035    __
$a (PubMed)21416290
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Illichmanová, Adéla. $7 _AN070377 $u Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500, Brno
245    10
$a Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen / $c A. Illichmanová, M. Janů, V. Sedláček, I. Kučera
520    9_
$a A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV·](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV·](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV·](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
650    _2
$a bakteriální proteiny $x chemie $x genetika $x metabolismus $7 D001426
650    _2
$a benzylviologen $x metabolismus $7 D001594
650    _2
$a transport elektronů $7 D004579
650    _2
$a kinetika $7 D007700
650    _2
$a nitrátreduktasa $x chemie $x genetika $x metabolismus $7 D050901
650    _2
$a dusičnany $x metabolismus $7 D009566
650    _2
$a oxidace-redukce $7 D010084
650    _2
$a Rhodobacteraceae $x chemie $x enzymologie $x genetika $7 D041902
650    _2
$a analýza jednotlivých buněk $7 D059010
655    _2
$a hodnotící studie $7 D023362
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1#
$a Janů, Miroslava. $7 _AN070378
700    1#
$a Sedláček, Vojtěch. $7 _AN067853
700    1_
$a Kučera, Igor, $d 1955- $7 ola2003204898
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 56, č. 1 (2011), s. 72-76
856    41
$u http://link.springer.com/journal/volumesAndIssues/12223 $y Pubmed
910    __
$a ABA008 $y m $b online $z 0
990    __
$a 20120817 $b ABA008
991    __
$a 20151021093924 $b ABA008
999    __
$a ok $b bmc $g 949748 $s 785052
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 56 $c 1 $d 72-76 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$b NLK111 $a Pubmed-20120817/11/03

Find record

Citation metrics

Archiving options