-
Something wrong with this record ?
Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells
H. Plchova, T. Moravec, H. Hoffmeisterova, J. Folwarczna, N. Cerovska,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- 3' Flanking Region MeSH
- 5' Flanking Region MeSH
- Agrobacterium tumefaciens MeSH
- Escherichia coli MeSH
- Gene Expression MeSH
- Papillomavirus Infections immunology virology MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- Human papillomavirus 16 genetics immunology metabolism MeSH
- Uterine Cervical Neoplasms immunology virology MeSH
- Papillomavirus E7 Proteins genetics immunology metabolism MeSH
- Potexvirus genetics immunology metabolism MeSH
- Protein Engineering methods MeSH
- Antibodies immunology MeSH
- Recombinant Fusion Proteins genetics immunology metabolism MeSH
- Nicotiana MeSH
- Papillomavirus Vaccines chemistry genetics MeSH
- Capsid Proteins genetics immunology metabolism MeSH
- Vaccines, Virus-Like Particle chemistry genetics MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The E7 oncoprotein from Human papillomavirus type 16 (HPV16) is an attractive candidate for anti-cancer therapeutical vaccine development. In this study, we engineered different fusions of mutagenized coding sequence of E7 oncoprotein (E7ggg) with coat protein of Potato virus X (PVX CP) both on 5'- and 3'-terminus of PVX CP and evaluated the influence of the length of linker (no linker, 4, 15aa) connecting PVX CP and E7ggg on their production. At first the expression in Escherichia coli was conducted to assess the characteristics of the recombinant protein prior to be further produced in plants, that is, resultant proteins were used for screening of their immunological reactivity with antibodies against PVX CP and E7. Fusion proteins successfully expressed in bacteria and plants were partially purified and their reactivity and ability to form virus-like particles were evaluated with anti-E7 antibodies.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12027856
- 003
- CZ-PrNML
- 005
- 20121210112921.0
- 007
- ta
- 008
- 120817e20110123xxu f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.pep.2011.01.008 $2 doi
- 035 __
- $a (PubMed)21266198
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Plchova, Helena $u Academy of Sciences of the Czech Republic, 160 00 Prague 6, Czech Republic.
- 245 10
- $a Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells / $c H. Plchova, T. Moravec, H. Hoffmeisterova, J. Folwarczna, N. Cerovska,
- 520 9_
- $a The E7 oncoprotein from Human papillomavirus type 16 (HPV16) is an attractive candidate for anti-cancer therapeutical vaccine development. In this study, we engineered different fusions of mutagenized coding sequence of E7 oncoprotein (E7ggg) with coat protein of Potato virus X (PVX CP) both on 5'- and 3'-terminus of PVX CP and evaluated the influence of the length of linker (no linker, 4, 15aa) connecting PVX CP and E7ggg on their production. At first the expression in Escherichia coli was conducted to assess the characteristics of the recombinant protein prior to be further produced in plants, that is, resultant proteins were used for screening of their immunological reactivity with antibodies against PVX CP and E7. Fusion proteins successfully expressed in bacteria and plants were partially purified and their reactivity and ability to form virus-like particles were evaluated with anti-E7 antibodies.
- 650 _2
- $a 3' přiléhající oblast DNA $7 D024509
- 650 _2
- $a 5' přiléhající oblast DNA $7 D024506
- 650 _2
- $a Agrobacterium tumefaciens $7 D016960
- 650 _2
- $a protilátky $x imunologie $7 D000906
- 650 _2
- $a virové plášťové proteiny $x genetika $x imunologie $x metabolismus $7 D036022
- 650 _2
- $a klonování DNA $7 D003001
- 650 _2
- $a Escherichia coli $7 D004926
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a exprese genu $7 D015870
- 650 _2
- $a lidský papilomavirus 16 $x genetika $x imunologie $x metabolismus $7 D052162
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a Papillomavirus E7 - proteiny $x genetika $x imunologie $x metabolismus $7 D050725
- 650 _2
- $a infekce papilomavirem $x imunologie $x virologie $7 D030361
- 650 _2
- $a vakcíny proti papilomavirům $x chemie $x genetika $7 D053918
- 650 _2
- $a Potexvirus $x genetika $x imunologie $x metabolismus $7 D017863
- 650 _2
- $a proteinové inženýrství $x metody $7 D015202
- 650 _2
- $a rekombinantní fúzní proteiny $x genetika $x imunologie $x metabolismus $7 D011993
- 650 _2
- $a tabák $7 D014026
- 650 _2
- $a nádory děložního čípku $x imunologie $x virologie $7 D002583
- 650 _2
- $a VLP vakcíny $x chemie $x genetika $7 D058425
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Moravec, Tomas
- 700 1_
- $a Hoffmeisterova, Hana
- 700 1_
- $a Folwarczna, Jitka
- 700 1_
- $a Cerovska, Noemi
- 773 0_
- $w MED00008659 $t Protein expression and purification $x 1096-0279 $g Roč. 77, č. 2 (20110123), s. 146-52
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21266198 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121210112958 $b ABA008
- 999 __
- $a ok $b bmc $g 949898 $s 785202
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 77 $c 2 $d 146-52 $e 20110123 $i 1096-0279 $m Protein expression and purification $n Protein Expr Purif $x MED00008659
- LZP __
- $a Pubmed-20120817/11/03
