-
Je něco špatně v tomto záznamu ?
Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathy
L. Pácal, J. Tomandl, J. Svojanovsky, D. Krusová, S. Stepánková, J. Rehorová, J. Olsovsky, J. Belobrádková, V. Tanhäuserová, M. Tomandlová, J. Muzík, K. Kanková,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1996 do Před 1 rokem
Open Access Digital Library
od 1996-01-01
PubMed
20826743
DOI
10.1093/ndt/gfq550
Knihovny.cz E-zdroje
- MeSH
- diabetické nefropatie enzymologie genetika mortalita MeSH
- dospělí MeSH
- erytrocyty enzymologie MeSH
- genotyp MeSH
- glukosa metabolismus MeSH
- jednonukleotidový polymorfismus genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- míra přežití MeSH
- následné studie MeSH
- pentosafosfáty metabolismus MeSH
- pentózofosfátový cyklus MeSH
- průřezové studie MeSH
- senioři MeSH
- thiamin metabolismus MeSH
- transaldolasa genetika MeSH
- transketolasa genetika MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Pentose phosphate pathway (PPP) represents a potentially 'protective' mechanism in hyperglycaemia due to shunting of glycolytic intermediates into PPP reactions. We hypothesized that thiamine status (plasma and erythrocyte levels of thiamine and its esters) together with genetic variability in key PPP enzymes-transketolase (TKT), transaldolase and TKT-like-might contribute to the progression of diabetic nephropathy (DN) and mortality of diabetics. METHODS: A total of 240 diabetic subjects with variable degree of kidney disease were included at baseline and were followed up for a median of 26 (IQR 21-50) months. Concentrations of thiamine in plasma and whole blood and TKT-catalysed reaction were determined by HPLC. Single-nucleotide polymorphisms (SNPs) (n = 14) were genotyped by means of PCR using TaqMan chemistry (Applied Biosystems, Foster City, CA, USA). RESULTS: Significant differences in pTh, pThDP, eryThDP and eryTKT between DN-stage groups were ascertained (P < 0.05) with advancing stage of DN being accompanied with increasing values of pTh, pThDP and eryTKT but not eryThDP. A highly significant negative correlation (r = - 0.41, P < 0.001) was found between pThDP and eryThDP, and the tertiles of the ratio of eryThDP/pThDP were significantly associated with all-cause mortality rates (P = 0.0072). We also identified significant differences in the rate of DN progression between different pTDP tertile groups (P = 0.0017). No significant genetic effects were found. CONCLUSIONS: The results support the role of 'functional' thiamine deficiency in the development of hyperglycaemia-related pathology. Limited intracellular availability of active TKT co-factor seems to be a dominant abnormality.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12027961
- 003
- CZ-PrNML
- 005
- 20121210094554.0
- 007
- ta
- 008
- 120817s2011 enk f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1093/ndt/gfq550 $2 doi
- 035 __
- $a (PubMed)20826743
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Pácal, Lukás $u Department of Pathophysiology, Masaryk University, Brno, Czech Republic.
- 245 10
- $a Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathy / $c L. Pácal, J. Tomandl, J. Svojanovsky, D. Krusová, S. Stepánková, J. Rehorová, J. Olsovsky, J. Belobrádková, V. Tanhäuserová, M. Tomandlová, J. Muzík, K. Kanková,
- 520 9_
- $a BACKGROUND: Pentose phosphate pathway (PPP) represents a potentially 'protective' mechanism in hyperglycaemia due to shunting of glycolytic intermediates into PPP reactions. We hypothesized that thiamine status (plasma and erythrocyte levels of thiamine and its esters) together with genetic variability in key PPP enzymes-transketolase (TKT), transaldolase and TKT-like-might contribute to the progression of diabetic nephropathy (DN) and mortality of diabetics. METHODS: A total of 240 diabetic subjects with variable degree of kidney disease were included at baseline and were followed up for a median of 26 (IQR 21-50) months. Concentrations of thiamine in plasma and whole blood and TKT-catalysed reaction were determined by HPLC. Single-nucleotide polymorphisms (SNPs) (n = 14) were genotyped by means of PCR using TaqMan chemistry (Applied Biosystems, Foster City, CA, USA). RESULTS: Significant differences in pTh, pThDP, eryThDP and eryTKT between DN-stage groups were ascertained (P < 0.05) with advancing stage of DN being accompanied with increasing values of pTh, pThDP and eryTKT but not eryThDP. A highly significant negative correlation (r = - 0.41, P < 0.001) was found between pThDP and eryThDP, and the tertiles of the ratio of eryThDP/pThDP were significantly associated with all-cause mortality rates (P = 0.0072). We also identified significant differences in the rate of DN progression between different pTDP tertile groups (P = 0.0017). No significant genetic effects were found. CONCLUSIONS: The results support the role of 'functional' thiamine deficiency in the development of hyperglycaemia-related pathology. Limited intracellular availability of active TKT co-factor seems to be a dominant abnormality.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a průřezové studie $7 D003430
- 650 _2
- $a diabetické nefropatie $x enzymologie $x genetika $x mortalita $7 D003928
- 650 _2
- $a erytrocyty $x enzymologie $7 D004912
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a následné studie $7 D005500
- 650 _2
- $a genotyp $7 D005838
- 650 _2
- $a glukosa $x metabolismus $7 D005947
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a pentózofosfátový cyklus $7 D010427
- 650 _2
- $a pentosafosfáty $x metabolismus $7 D010428
- 650 _2
- $a jednonukleotidový polymorfismus $x genetika $7 D020641
- 650 _2
- $a míra přežití $7 D015996
- 650 _2
- $a thiamin $x metabolismus $7 D013831
- 650 _2
- $a transaldolasa $x genetika $7 D014153
- 650 _2
- $a transketolasa $x genetika $7 D014174
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Tomandl, Josef
- 700 1_
- $a Svojanovsky, Jan
- 700 1_
- $a Krusová, Darja
- 700 1_
- $a Stepánková, Sona
- 700 1_
- $a Rehorová, Jitka
- 700 1_
- $a Olsovsky, Jindrich
- 700 1_
- $a Belobrádková, Jana
- 700 1_
- $a Tanhäuserová, Veronika
- 700 1_
- $a Tomandlová, Marie
- 700 1_
- $a Muzík, Jan
- 700 1_
- $a Kanková, Katerina
- 773 0_
- $w MED00010288 $t Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association $x 1460-2385 $g Roč. 26, č. 4 (2011), s. 1229-36
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/20826743 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121210094631 $b ABA008
- 999 __
- $a ok $b bmc $g 950003 $s 785307
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 26 $c 4 $d 1229-36 $i 1460-2385 $m Nephrology, dialysis, transplantation $n Nephrol Dial Transplant $x MED00010288
- LZP __
- $a Pubmed-20120817/11/03
