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Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC
L. Krcmova, D. Solichova, B. Melichar, M. Kasparova, J. Plisek, L. Sobotka, P. Solich
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Phosphates chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Creatinine blood MeSH
- Kynurenine blood MeSH
- Humans MeSH
- Neopterin blood MeSH
- Buffers MeSH
- Reproducibility of Results MeSH
- Tryptophan blood MeSH
- Chromatography, High Pressure Liquid instrumentation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
References provided by Crossref.org
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- $a Krčmová Kujovská, Lenka, $7 xx0209338 $u Department of Metabolic Care and Gerontology, University Hospital, Hradec Kralove, Czech Republic. Lenkakrcmova@seznam.cz $d 1981-
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- $a Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC / $c L. Krcmova, D. Solichova, B. Melichar, M. Kasparova, J. Plisek, L. Sobotka, P. Solich
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- $a A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
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