Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Effect of soman on JNK and p38 mitogen activated protein kinase (MAPK) pathways

Jaroslav Pejchal, Jan Österreicher, Jiri Kassa, Vaclav Marak, Ales Tichy, Zuzana Sinkorova, Lenka Zarybnicka, Klara Kubelkova and Kamil Kuca

. 2012 ; 81 (2) : 68-75.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc12028679

The purpose of our study was to examine an early activation of JNK and p38 mitogen activated protein kinases (MAPK) and their substrate c-Myc after soman poisoning in order to enlighten the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were intramuscularly poisoned by soman (60 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-JNKThr-183/Tyr-185, phospho-p38Thr180/Tyr182, and phospho-c- MycThr58/Ser62 expressions were measured using a computer Image analysis in apical and cryptal enterocytes of the colon transversum. We observed decreased phospho-JNK in apical enterocytes 4 and 24 h after poisoning and increased phospho-JNK in cryptal and apical enterocytes 72 h after intoxication. Phosphop38 dropped significantly in the apical compartment 72 h after soman poisoning. An activation of c-Myc decreased in both apical and cryptal compartment 4 and 24 h after soman intoxication, while increased in both compartments 72 h after poisoning. Soman poisoning seems to temporarily suppress promitotic pathways of proliferating cryptal cells and causes delayed activation of JNK stress signaling pathway.

Citace poskytuje Crossref.org

Obsahuje 3 tabulky

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc12028679
003      
CZ-PrNML
005      
20121009091737.0
007      
ta
008      
120830s2012 xr f f 000 0eng||
009      
AR
024    7_
$a 10.31482/mmsl.2012.009 $2 doi
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Pejchal, Jaroslav $7 xx0102560 $u Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
245    10
$a Effect of soman on JNK and p38 mitogen activated protein kinase (MAPK) pathways / $c Jaroslav Pejchal, Jan Österreicher, Jiri Kassa, Vaclav Marak, Ales Tichy, Zuzana Sinkorova, Lenka Zarybnicka, Klara Kubelkova and Kamil Kuca
500    __
$a Obsahuje 3 tabulky
504    __
$a Literatura $b 36
520    9_
$a The purpose of our study was to examine an early activation of JNK and p38 mitogen activated protein kinases (MAPK) and their substrate c-Myc after soman poisoning in order to enlighten the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were intramuscularly poisoned by soman (60 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-JNKThr-183/Tyr-185, phospho-p38Thr180/Tyr182, and phospho-c- MycThr58/Ser62 expressions were measured using a computer Image analysis in apical and cryptal enterocytes of the colon transversum. We observed decreased phospho-JNK in apical enterocytes 4 and 24 h after poisoning and increased phospho-JNK in cryptal and apical enterocytes 72 h after intoxication. Phosphop38 dropped significantly in the apical compartment 72 h after soman poisoning. An activation of c-Myc decreased in both apical and cryptal compartment 4 and 24 h after soman intoxication, while increased in both compartments 72 h after poisoning. Soman poisoning seems to temporarily suppress promitotic pathways of proliferating cryptal cells and causes delayed activation of JNK stress signaling pathway.
650    _2
$a financování organizované $7 D005381
650    _2
$a zvířata $7 D000818
650    _2
$a experimenty na zvířatech $7 D032761
650    _2
$a potkani Wistar $7 D017208
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a výzkumný projekt $7 D012107
650    _2
$a kontrolní skupiny $7 D035061
650    _2
$a časové faktory $7 D013997
650    _2
$a soman $x otrava $x toxicita $7 D012999
650    _2
$a JNK mitogenem aktivované proteinkinasy $x chemie $7 D048031
650    _2
$a mitogenem aktivované proteinkinasy p38 $x chemie $7 D048051
650    _2
$a colon transversum $x chemie $7 D044684
650    _2
$a enterocyty $x chemie $7 D020895
650    _2
$a MAP kinasový signální systém $x fyziologie $7 D020935
650    _2
$a protoonkogenní proteiny c-myc $x chemie $7 D016271
700    1_
$a Österreicher, Jan, $d 1971- $7 jn20020726047 $u Department of Radiation Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Kassa, Jiří, $d 1956- $7 mzk2003181395 $u Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Mařák, Václav. $7 _AN068974 $u Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Tichý, Aleš, $d 1980- $7 xx0135624 $u Department of Radiation Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Šinkorová, Zuzana, $d 1963- $7 xx0106471 $u Department of Radiation Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Zárybnická, Lenka $7 xx0135626 $u Department of Radiation Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Kubelková, Klára. $7 hka2017949672 $u Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
700    1_
$a Kuča, Kamil, $d 1978- $7 xx0041831 $u Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
773    0_
$t Vojenské zdravotnické listy $x 0372-7025 $g Roč. 81, č. 2 (2012), s. 68-75 $w MED00011116
910    __
$a ABA008 $b A 3 $c 1073 $y 2
990    __
$a 20120830071444 $b ABA008
991    __
$a 20121009091929 $b ABA008
999    __
$a ok $b bmc $g 950742 $s 786031
BAS    __
$a 3
BMC    __
$a 2012 $b 81 $c 2 $d 68-75 $i 0372-7025 $m Vojenské zdravotnické listy $n Vojen. zdr. listy $x MED00011116
LZP    __
$a 2012-33/vthv

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace