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Recognition of avirulence gene AvrLm1 from hemibiotrophic ascomycete Leptosphaeria maculans triggers salicylic acid and ethylene signaling in Brassica napus
V. Sašek, M. Nováková, B. Jindřichová, K. Bóka, O. Valentová, L. Burketová
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1988 to 1 year ago
Freely Accessible Science Journals
from 1988 to 1 year ago
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from 1988
- MeSH
- Ascomycota metabolism MeSH
- Brassica napus drug effects metabolism microbiology MeSH
- Time Factors MeSH
- Ethylenes metabolism MeSH
- Fungal Proteins metabolism pharmacology MeSH
- Salicylic Acid metabolism MeSH
- Plant Leaves drug effects metabolism microbiology MeSH
- Plant Diseases MeSH
- Gene Expression Regulation, Fungal MeSH
- Signal Transduction physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Interaction of a plant with a fungal pathogen is an encounter with hundreds of molecules. In contrast to this, a single molecule often decides between the disease and resistance. In the present article, we describe the defense responses triggered by AvrLm1, an avirulence gene from a hemibiotrophic ascomycete, Leptosphaeria maculans, responsible for an incompatible interaction with Brassica napus. Using multiple hormone quantification and expression analysis of defense-related genes, we investigated signaling events in Rlm1 plants infected with two sister isolates of L. maculans differentiated by the presence or absence of AvrLm1. Infection with the isolate carrying AvrLm1 increased the biosynthesis of salicylic acid (SA) and induced expression of the SA-associated genes ICS1, WRKY70, and PR-1, a feature characteristic of responses to biotrophic pathogens and resistance gene-mediated resistance. In addition to SA-signaling elements, we also observed the induction of ASC2a, HEL, and CHI genes associated with ethylene (ET) signaling. Pharmacological experiments confirmed the positive roles of SA and ET in mediating resistance to L. maculans. The unusual cooperation of SA and ET signaling might be a response to the hemibiotrophic nature of L. maculans. Our results also demonstrate the profound difference between the natural host B. napus and the model plant Arabidopsis in their response to L. maculans infection.
Academy of Sciences of the Czech Republic Prague Czech Republic
Department of Plant Anatomy Eötvös Loránd University Pázmány Péter sétány 1 C H 1117 Hungary
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