• Je něco špatně v tomto záznamu ?

Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction

M. Magyar, G. Sipka, L. Kovács, B. Ughy, Q. Zhu, G. Han, V. Špunda, PH. Lambrev, JR. Shen, G. Garab,

. 2018 ; 8 (1) : 2755. [pub] 20180209

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035406

Photosystem II (PSII) catalyses the photoinduced oxygen evolution and, by producing reducing equivalents drives, in concert with PSI, the conversion of carbon dioxide to sugars. Our knowledge about the architecture of the reaction centre (RC) complex and the mechanisms of charge separation and stabilisation is well advanced. However, our understanding of the processes associated with the functioning of RC is incomplete: the photochemical activity of PSII is routinely monitored by chlorophyll-a fluorescence induction but the presently available data are not free of controversy. In this work, we examined the nature of gradual fluorescence rise of PSII elicited by trains of single-turnover saturating flashes (STSFs) in the presence of a PSII inhibitor, permitting only one stable charge separation. We show that a substantial part of the fluorescence rise originates from light-induced processes that occur after the stabilisation of charge separation, induced by the first STSF; the temperature-dependent relaxation characteristics suggest the involvement of conformational changes in the additional rise. In experiments using double flashes with variable waiting times (∆τ) between them, we found that no rise could be induced with zero or short ∆τ, the value of which depended on the temperature - revealing a previously unknown rate-limiting step in PSII.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035406
003      
CZ-PrNML
005      
20191008113031.0
007      
ta
008      
191007s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-018-21195-2 $2 doi
035    __
$a (PubMed)29426901
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Magyar, Melinda $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary.
245    10
$a Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction / $c M. Magyar, G. Sipka, L. Kovács, B. Ughy, Q. Zhu, G. Han, V. Špunda, PH. Lambrev, JR. Shen, G. Garab,
520    9_
$a Photosystem II (PSII) catalyses the photoinduced oxygen evolution and, by producing reducing equivalents drives, in concert with PSI, the conversion of carbon dioxide to sugars. Our knowledge about the architecture of the reaction centre (RC) complex and the mechanisms of charge separation and stabilisation is well advanced. However, our understanding of the processes associated with the functioning of RC is incomplete: the photochemical activity of PSII is routinely monitored by chlorophyll-a fluorescence induction but the presently available data are not free of controversy. In this work, we examined the nature of gradual fluorescence rise of PSII elicited by trains of single-turnover saturating flashes (STSFs) in the presence of a PSII inhibitor, permitting only one stable charge separation. We show that a substantial part of the fluorescence rise originates from light-induced processes that occur after the stabilisation of charge separation, induced by the first STSF; the temperature-dependent relaxation characteristics suggest the involvement of conformational changes in the additional rise. In experiments using double flashes with variable waiting times (∆τ) between them, we found that no rise could be induced with zero or short ∆τ, the value of which depended on the temperature - revealing a previously unknown rate-limiting step in PSII.
650    _2
$a chlorofyl a $x metabolismus $7 D000077194
650    12
$a fluorescence $7 D005453
650    _2
$a fotosyntéza $7 D010788
650    _2
$a fotosystém II - proteinový komplex $x antagonisté a inhibitory $x metabolismus $7 D045332
650    _2
$a Spinacia oleracea $x metabolismus $7 D018724
650    _2
$a Synechococcus $x metabolismus $7 D046940
650    _2
$a Synechocystis $x metabolismus $7 D046939
650    _2
$a teplota $7 D013696
650    _2
$a tylakoidy $x metabolismus $7 D020524
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Sipka, Gábor $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary.
700    1_
$a Kovács, László $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary.
700    1_
$a Ughy, Bettina $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary.
700    1_
$a Zhu, Qingjun $u Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany the Chinese Academy of Sciences, Beijing, 100093, China.
700    1_
$a Han, Guangye $u Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany the Chinese Academy of Sciences, Beijing, 100093, China.
700    1_
$a Špunda, Vladimír $u Department of Physics, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00, Ostrava, Czech Republic. Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, 603 00, Brno, Czech Republic.
700    1_
$a Lambrev, Petar H $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary.
700    1_
$a Shen, Jian-Ren $u Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany the Chinese Academy of Sciences, Beijing, 100093, China. Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, 1-1, Naka 3-chome, Tsushima, Okayama, 700-8530, Japan.
700    1_
$a Garab, Győző $u Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, H-6726, Szeged, Hungary. garab.gyozo@brc.mta.hu. Department of Physics, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00, Ostrava, Czech Republic. garab.gyozo@brc.mta.hu.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 8, č. 1 (2018), s. 2755
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29426901 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191008113447 $b ABA008
999    __
$a ok $b bmc $g 1452066 $s 1073956
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 8 $c 1 $d 2755 $e 20180209 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20191007

Najít záznam

Citační ukazatele

Možnosti archivace