• Je něco špatně v tomto záznamu ?

Live imaging of mitosomes and hydrogenosomes by HaloTag technology

E. Martincová, L. Voleman, V. Najdrová, M. De Napoli, S. Eshar, M. Gualdron, CS. Hopp, DE. Sanin, DL. Tembo, D. Van Tyne, D. Walker, M. Marcinčiková, J. Tachezy, P. Doležal

. 2012 ; 7 (4) : e36314.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12034527

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12034527
003      
CZ-PrNML
005      
20210216155942.0
007      
ta
008      
121023s2012 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0036314 $2 doi
035    __
$a (PubMed)22558433
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Martincová, Eva $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
245    10
$a Live imaging of mitosomes and hydrogenosomes by HaloTag technology / $c E. Martincová, L. Voleman, V. Najdrová, M. De Napoli, S. Eshar, M. Gualdron, CS. Hopp, DE. Sanin, DL. Tembo, D. Van Tyne, D. Walker, M. Marcinčiková, J. Tachezy, P. Doležal
520    9_
$a Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
650    _2
$a anaerobióza $7 D000693
650    _2
$a viabilita buněk $7 D002470
650    _2
$a reportérové geny $x genetika $7 D017930
650    _2
$a genetické vektory $x genetika $7 D005822
650    _2
$a Giardia lamblia $x cytologie $x genetika $7 D016829
650    _2
$a hydrolasy $x genetika $7 D006867
650    _2
$a ligandy $7 D008024
650    _2
$a mitochondrie $x metabolismus $7 D008928
650    _2
$a molekulární zobrazování $x metody $7 D057054
650    _2
$a organely $x metabolismus $7 D015388
650    _2
$a protozoální proteiny $x genetika $7 D015800
650    _2
$a rekombinantní fúzní proteiny $x genetika $7 D011993
650    _2
$a Trichomonas vaginalis $x cytologie $x genetika $7 D014246
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Voleman, Luboš $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Najdrová, Vladimíra $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a De Napoli, Maximiliano $u Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires, Argentina
700    1_
$a Eshar, Shiri $u Department of Microbiology and Molecular Genetics, The Kuvin Center for the Study of Infectious and Tropical Diseases, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
700    1_
$a Gualdron, Melisa $u Christian De Duve Institute of Cellular Pathology, Brussels, Belgium
700    1_
$a Hopp, Christine S. $u Malaria Centre, London School of Hygiene & Tropical Medicine, London, United Kingdom
700    1_
$a Sanin, David E. $u Department of Biology, University of York, York, United Kingdom
700    1_
$a Tembo, Dumizulu L. $u Malawi-Liverpool Wellcome Trust Clinical Research Programme, Chichiri, Blantyre, Malawi
700    1_
$a Van Tyne, Daria $u Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, United States of America
700    1_
$a Walker, Dawn $u Integrated Biomedical Sciences Center for Microbial Interface Biology Department of Internal Medicine, Columbus, Ohio, United States of America
700    1_
$a Marcinčiková, Michaela $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Tachezy, Jan, $d 1958- $7 mzk2008442769 $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Doležal, Pavel, $7 xx0257242 $d 1975- $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
773    0_
$w MED00180950 $t PLoS ONE $x 1932-6203 $g Roč. 7, č. 4 (2012), s. e36314
856    41
$u https://pubmed.ncbi.nlm.nih.gov/22558433 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20121023 $b ABA008
991    __
$a 20210216155939 $b ABA008
999    __
$a ok $b bmc $g 956537 $s 792024
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2012 $b 7 $c 4 $d e36314 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$b NLK112 $a Pubmed-20121023

Najít záznam

Citační ukazatele

Možnosti archivace