-
Something wrong with this record ?
Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure
V. Burkina, G. Zamaratskaia, T. Randak, ZH. Li, G. Fedorova, J. Pickova, V. Zlabek,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biomarkers metabolism MeSH
- Calcium Channel Blockers toxicity MeSH
- Water Pollutants, Chemical toxicity MeSH
- Microsomes, Liver drug effects enzymology metabolism MeSH
- Liver drug effects enzymology metabolism MeSH
- Coumarins metabolism MeSH
- Nitrophenols metabolism MeSH
- Oncorhynchus mykiss MeSH
- Oxazines metabolism MeSH
- Cytochrome P-450 Enzyme System metabolism MeSH
- Verapamil toxicity MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Little is known about the effects of the cardiovascular drug verapamil (VRP) on metabolic processes in fish. Most calcium channel blockers including VRP are metabolized by cytochrome P450 (CYP450) enzymes. In this study we investigated the in vivo effect of VRP on some CYP450-mediated reactions in juvenile rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of VRP (0.5, 27 and 270 μg l(-1)) for 0, 21, and 42 day. The following CYP450-mediated reactions were studied in hepatic microsomes: O-dealkylation of ethoxyresorufin, methoxyresorufin, and pentoxyresorufin, hydroxylation of coumarin, tolbutamide, and p-nitrophenol, and O-debenzylation of 7-benzyloxy-4-trifluoromethylcoumarin. The amounts of products of these reactions did not differ among fish exposed to different levels of VRP and control fish. This suggests that the levels of VPR used did not alter catalytic activity of the selected CYP450 enzymes. In conclusion, none of the investigated CYP450-mediated reactions has potential as a biomarker to monitor VRP contamination of the aquatic environment.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12034794
- 003
- CZ-PrNML
- 005
- 20121105113211.0
- 007
- ta
- 008
- 121023s2012 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.ecoenv.2011.12.012 $2 doi
- 035 __
- $a (PubMed)22244749
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Burkina, V $u University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic. vburkina@frov.jcu.cz
- 245 10
- $a Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure / $c V. Burkina, G. Zamaratskaia, T. Randak, ZH. Li, G. Fedorova, J. Pickova, V. Zlabek,
- 520 9_
- $a Little is known about the effects of the cardiovascular drug verapamil (VRP) on metabolic processes in fish. Most calcium channel blockers including VRP are metabolized by cytochrome P450 (CYP450) enzymes. In this study we investigated the in vivo effect of VRP on some CYP450-mediated reactions in juvenile rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of VRP (0.5, 27 and 270 μg l(-1)) for 0, 21, and 42 day. The following CYP450-mediated reactions were studied in hepatic microsomes: O-dealkylation of ethoxyresorufin, methoxyresorufin, and pentoxyresorufin, hydroxylation of coumarin, tolbutamide, and p-nitrophenol, and O-debenzylation of 7-benzyloxy-4-trifluoromethylcoumarin. The amounts of products of these reactions did not differ among fish exposed to different levels of VRP and control fish. This suggests that the levels of VPR used did not alter catalytic activity of the selected CYP450 enzymes. In conclusion, none of the investigated CYP450-mediated reactions has potential as a biomarker to monitor VRP contamination of the aquatic environment.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biologické markery $x metabolismus $7 D015415
- 650 _2
- $a blokátory kalciových kanálů $x toxicita $7 D002121
- 650 _2
- $a kumariny $x metabolismus $7 D003374
- 650 _2
- $a systém (enzymů) cytochromů P-450 $x metabolismus $7 D003577
- 650 _2
- $a vztah mezi dávkou a účinkem léčiva $7 D004305
- 650 _2
- $a játra $x účinky léků $x enzymologie $x metabolismus $7 D008099
- 650 _2
- $a jaterní mikrozomy $x účinky léků $x enzymologie $x metabolismus $7 D008862
- 650 _2
- $a nitrofenoly $x metabolismus $7 D009596
- 650 _2
- $a Oncorhynchus mykiss $7 D017686
- 650 _2
- $a oxaziny $x metabolismus $7 D010078
- 650 _2
- $a verapamil $x toxicita $7 D014700
- 650 _2
- $a chemické látky znečišťující vodu $x toxicita $7 D014874
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zamaratskaia, G
- 700 1_
- $a Randák, Tomáš, $d 1975- $7 mzk2006363431
- 700 1_
- $a Li, Z H
- 700 1_
- $a Fedorova, G
- 700 1_
- $a Pickova, J
- 700 1_
- $a Žlábek, Vladimír $7 xx0097941
- 773 0_
- $w MED00001478 $t Ecotoxicology and environmental safety $x 1090-2414 $g Roč. 79(2012), s. 148-52
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22244749 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a
- 990 __
- $a 20121023 $b ABA008
- 991 __
- $a 20121105113219 $b ABA008
- 999 __
- $a ok $b bmc $g 956804 $s 792291
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 79 $d 148-52 $i 1090-2414 $m Ecotoxicology and environmental safety $n Ecotoxicol Environ Safety $x MED00001478
- LZP __
- $a Pubmed-20121023
