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Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
M. Varga, L. Kuntová, R. Pantůček, I. Mašlaňová, V. Růžičková, J. Doškař,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu dopisy, práce podpořená grantem
NLK
ProQuest Central
od 1996-01-01 do 2012-12-31
Medline Complete (EBSCOhost)
od 2006-01-01 do 2014-12-15
Health & Medicine (ProQuest)
od 1996-01-01 do 2012-12-31
Wiley Online Library (archiv)
od 1997-01-01 do 2012-12-31
Public Health Database (ProQuest)
od 1996-01-01 do 2012-12-31
- MeSH
- bakteriální léková rezistence MeSH
- bakteriofágy genetika MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus genetika izolace a purifikace virologie MeSH
- plazmidy MeSH
- přenos genů horizontální MeSH
- profágy genetika MeSH
- stafylokokové infekce mikrobiologie MeSH
- transdukce genetická MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- dopisy MeSH
- práce podpořená grantem MeSH
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages φ80α and φJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10(-5) - 10(-6) CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1 : 1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone.
Citace poskytuje Crossref.org
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- $a The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages φ80α and φJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10(-5) - 10(-6) CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1 : 1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone.
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