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Comparison of "high throughput" micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification

E. Anzenbacherová, A. Veinlichová, V. Masek, P. Anzenbacher

. 2005 ; 149 (2) : 353-355.

Jazyk angličtina Země Česko

Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc13003741

Enzyme activities of the CYP enzymes (CYP3A4, CYP2C9 and CYP2A6) were determined using classical substrates (testosterone, diclofenac and coumarin, respectively) as well as with luminogenic or fluorogenic substrates in micromethod arrangement. The luciferin-based luminogenic substrates for CYP3A4 and CYP2C9 as well as coumarin in micromethod for assay of CYP2A6 activity gave results well comparable with the classical methods with determination of reaction products by the HPLC.

Citace poskytuje Crossref.org

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