-
Something wrong with this record ?
Structure of rrn operons in pathogenic non-cultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
D. Cejková, M. Zobaníková, P. Pospísilová, M. Strouhal, L. Mikalová, GM. Weinstock, D. Smajs,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT11159
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 1968 to 1 year ago
- MeSH
- DNA, Bacterial chemistry genetics MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Genome, Bacterial MeSH
- Genotype MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Molecular Sequence Data MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- rRNA Operon * MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Deletion MeSH
- Treponema pallidum classification genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S-23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S-23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13012388
- 003
- CZ-PrNML
- 005
- 20170418101651.0
- 007
- ta
- 008
- 130404s2013 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1099/jmm.0.050658-0 $2 doi
- 035 __
- $a (PubMed)23082031
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Čejková, Darina, $u Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 62500 Brno, Czech Republic. $d 1978- $7 mzk2010590837
- 245 10
- $a Structure of rrn operons in pathogenic non-cultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains / $c D. Cejková, M. Zobaníková, P. Pospísilová, M. Strouhal, L. Mikalová, GM. Weinstock, D. Smajs,
- 520 9_
- $a This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S-23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S-23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
- 650 _2
- $a sekvence nukleotidů $7 D001483
- 650 _2
- $a DNA bakterií $x chemie $x genetika $7 D004269
- 650 _2
- $a mezerníky ribozomální DNA $x genetika $7 D021903
- 650 _2
- $a genetická variace $7 D014644
- 650 _2
- $a genom bakteriální $7 D016680
- 650 _2
- $a genotyp $7 D005838
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a RNA ribozomální 16S $x genetika $7 D012336
- 650 _2
- $a RNA ribozomální 23S $x genetika $7 D012338
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 _2
- $a sekvenční delece $7 D017384
- 650 _2
- $a Treponema pallidum $x klasifikace $x genetika $7 D014210
- 650 12
- $a rRNA Operon $7 D012407
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zobaníková, Marie $7 mzk2013794986
- 700 1_
- $a Pospíšilová, Petra, $d 1978- $7 _AN056750
- 700 1_
- $a Strouhal, Michal $7 xx0085514
- 700 1_
- $a Mikalová, Lenka $7 xx0199108
- 700 1_
- $a Weinstock, George M $u -
- 700 1_
- $a Šmajs, David, $d 1969- $7 xx0061318
- 773 0_
- $w MED00002792 $t Journal of medical microbiology $x 1473-5644 $g Roč. 62, č. Pt 2 (2013), s. 196-207
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/23082031 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130404 $b ABA008
- 991 __
- $a 20170418101959 $b ABA008
- 999 __
- $a ok $b bmc $g 975586 $s 810669
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 62 $c Pt 2 $d 196-207 $i 1473-5644 $m Journal of Medical Microbiology $n J Med Microbiol $x MED00002792
- GRA __
- $a NT11159 $p MZ0
- LZP __
- $a Pubmed-20130404