-
Je něco špatně v tomto záznamu ?
Amniotic fluid cathelicidin in PPROM pregnancies: from proteomic discovery to assessing its potential in inflammatory complications diagnosis
V. Tambor, M. Kacerovsky, C. Andrys, I. Musilova, H. Hornychova, L. Pliskova, M. Link, J. Stulik, J. Lenco,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
Free Medical Journals
od 2006
Public Library of Science (PLoS)
od 2006
PubMed Central
od 2006
Europe PubMed Central
od 2006
ProQuest Central
od 2006-12-01
Open Access Digital Library
od 2006-01-01
Open Access Digital Library
od 2006-10-01
Open Access Digital Library
od 2006-01-01
Medline Complete (EBSCOhost)
od 2008-01-01
Nursing & Allied Health Database (ProQuest)
od 2006-12-01
Health & Medicine (ProQuest)
od 2006-12-01
Public Health Database (ProQuest)
od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources
od 2006
- MeSH
- chorioamnionitida diagnóza metabolismus MeSH
- dospělí MeSH
- ELISA metody MeSH
- gestační stáří MeSH
- kationické antimikrobiální peptidy biosyntéza MeSH
- koncentrace vodíkových iontů MeSH
- leukocyty metabolismus MeSH
- lidé MeSH
- neutrofily metabolismus MeSH
- peptidy chemie MeSH
- plodová voda metabolismus MeSH
- pravděpodobnostní funkce MeSH
- předčasný odtok plodové vody diagnóza metabolismus mikrobiologie MeSH
- proteomika metody MeSH
- ROC křivka MeSH
- senzitivita a specificita MeSH
- těhotenství MeSH
- trypsin chemie MeSH
- zánět metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Preterm prelabor rupture of membranes (PPROM) complicated by microbial invasion of the amniotic cavity (MIAC) leading to histological chorioamnionitis (HCA) significantly impacts perinatal morbidity. Unfortunately, no well-established tool for identifying PPROM patients threatened by these disorders is available. METHODOLOGY/PRINCIPAL FINDINGS: We performed an unbiased exploratory analysis of amniotic fluid proteome changes due to MIAC and HCA. From among the top five proteins that showed the most profound and significant change, we sought to confirm results concerning cathelicidin (P49913, CAMP_HUMAN), since an ELISA kit was readily available for this protein. In our exploratory proteomic study, cathelicidin showed a ∼6-fold higher concentration in PPROM patients with confirmed MIAC and HCA. We verified significantly higher levels of cathelicidin in exploratory samples (women without both MIAC and HCA: median 1.4 ng/ml; women with both conditions confirmed: median 3.6 ng/ml; p = 0.0003). A prospective replication cohort was used for independent validation and for assessment of cathelicidin potential to stratify women with MIAC leading to HCA from women in whom at least one of these conditions was ruled out. We confirmed the association of higher amniotic fluid cathelicidin levels with MIAC leading to HCA (the presence of both MIAC and HCA: median 3.1 ng/ml; other women: median 1.4 ng/ml; p<0.0001). A cathelicidin concentration of 4.0 ng/ml was found to be the best cut-off point for identifying PPROM women with both MIAC and HCA. When tested on the validation cohort, a sensitivity of 48%, a specificity of 90%, a likelihood ratio of 5.0, and an area under receiver-operating characteristic curve of 71% were achieved for identification of women with MIAC leading to HCA. CONCLUSIONS: Our multi-stage study suggests cathelicidin as a candidate marker that should be considered for a panel of amniotic fluid proteins permitting identification of PPROM women with MIAC leading to HCA.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13012595
- 003
- CZ-PrNML
- 005
- 20130408112438.0
- 007
- ta
- 008
- 130404s2012 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1371/journal.pone.0041164 $2 doi
- 035 __
- $a (PubMed)22815956
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Tambor, Vojtech $u Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
- 245 10
- $a Amniotic fluid cathelicidin in PPROM pregnancies: from proteomic discovery to assessing its potential in inflammatory complications diagnosis / $c V. Tambor, M. Kacerovsky, C. Andrys, I. Musilova, H. Hornychova, L. Pliskova, M. Link, J. Stulik, J. Lenco,
- 520 9_
- $a BACKGROUND: Preterm prelabor rupture of membranes (PPROM) complicated by microbial invasion of the amniotic cavity (MIAC) leading to histological chorioamnionitis (HCA) significantly impacts perinatal morbidity. Unfortunately, no well-established tool for identifying PPROM patients threatened by these disorders is available. METHODOLOGY/PRINCIPAL FINDINGS: We performed an unbiased exploratory analysis of amniotic fluid proteome changes due to MIAC and HCA. From among the top five proteins that showed the most profound and significant change, we sought to confirm results concerning cathelicidin (P49913, CAMP_HUMAN), since an ELISA kit was readily available for this protein. In our exploratory proteomic study, cathelicidin showed a ∼6-fold higher concentration in PPROM patients with confirmed MIAC and HCA. We verified significantly higher levels of cathelicidin in exploratory samples (women without both MIAC and HCA: median 1.4 ng/ml; women with both conditions confirmed: median 3.6 ng/ml; p = 0.0003). A prospective replication cohort was used for independent validation and for assessment of cathelicidin potential to stratify women with MIAC leading to HCA from women in whom at least one of these conditions was ruled out. We confirmed the association of higher amniotic fluid cathelicidin levels with MIAC leading to HCA (the presence of both MIAC and HCA: median 3.1 ng/ml; other women: median 1.4 ng/ml; p<0.0001). A cathelicidin concentration of 4.0 ng/ml was found to be the best cut-off point for identifying PPROM women with both MIAC and HCA. When tested on the validation cohort, a sensitivity of 48%, a specificity of 90%, a likelihood ratio of 5.0, and an area under receiver-operating characteristic curve of 71% were achieved for identification of women with MIAC leading to HCA. CONCLUSIONS: Our multi-stage study suggests cathelicidin as a candidate marker that should be considered for a panel of amniotic fluid proteins permitting identification of PPROM women with MIAC leading to HCA.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a plodová voda $x metabolismus $7 D000653
- 650 _2
- $a kationické antimikrobiální peptidy $x biosyntéza $7 D023181
- 650 _2
- $a chorioamnionitida $x diagnóza $x metabolismus $7 D002821
- 650 _2
- $a ELISA $x metody $7 D004797
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a předčasný odtok plodové vody $x diagnóza $x metabolismus $x mikrobiologie $7 D005322
- 650 _2
- $a gestační stáří $7 D005865
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a koncentrace vodíkových iontů $7 D006863
- 650 _2
- $a zánět $x metabolismus $7 D007249
- 650 _2
- $a leukocyty $x metabolismus $7 D007962
- 650 _2
- $a pravděpodobnostní funkce $7 D016013
- 650 _2
- $a neutrofily $x metabolismus $7 D009504
- 650 _2
- $a peptidy $x chemie $7 D010455
- 650 _2
- $a těhotenství $7 D011247
- 650 _2
- $a proteomika $x metody $7 D040901
- 650 _2
- $a ROC křivka $7 D012372
- 650 _2
- $a senzitivita a specificita $7 D012680
- 650 _2
- $a trypsin $x chemie $7 D014357
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kacerovsky, Marian $u -
- 700 1_
- $a Andrýs, Ctirad $u - $7 mzk2008430528
- 700 1_
- $a Musilova, Ivana $u -
- 700 1_
- $a Hornychova, Helena $u -
- 700 1_
- $a Pliskova, Lenka $u -
- 700 1_
- $a Link, Marek $u -
- 700 1_
- $a Stulik, Jiri $u -
- 700 1_
- $a Lenco, Juraj $u -
- 773 0_
- $w MED00180950 $t PloS one $x 1932-6203 $g Roč. 7, č. 7 (2012), s. e41164
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22815956 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130404 $b ABA008
- 991 __
- $a 20130408112705 $b ABA008
- 999 __
- $a ok $b bmc $g 975793 $s 810876
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 7 $c 7 $d e41164 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
- LZP __
- $a Pubmed-20130404