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Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment
I. Sedláček, P. Holochová, I. Mašlaňová, M. Kosina, C. Spröer, H. Bryndová, P. Vandamme, I. Rudolf, Z. Hubálek, P. Švec,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1951 to 2 years ago
Freely Accessible Science Journals
from 2000 to 1 year ago
- MeSH
- Genes, Bacterial MeSH
- DNA, Bacterial genetics MeSH
- Enterococcus classification genetics isolation & purification MeSH
- Phylogeny * MeSH
- Nucleic Acid Hybridization MeSH
- Environmental Microbiology MeSH
- Molecular Sequence Data MeSH
- Drinking Water microbiology MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Urease biosynthesis MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629(T) = LMG 26676(T) = CCUG 48799(T)), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630(T) = LMG 26678(T) = CCUG 61593(T)), inhabiting water, insects (mosquitoes) and plants, are proposed.
References provided by Crossref.org
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