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The effect of immunosuppression on manifestations of sepsis in an animal model of cecal ligation and puncture
E. Kieslichova, M. Rocen, D. Merta, M. Kudla, I. Splichal, J. Cap, O. Viklicky, R. Gürlich
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
NS10556
MZ0
CEP - Centrální evidence projektů
- MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- cékum mikrobiologie chirurgie MeSH
- cyklosporin farmakologie MeSH
- hemodynamika MeSH
- imunokompromitovaný pacient * MeSH
- imunosupresiva farmakologie MeSH
- kardiotonika farmakologie MeSH
- kyselina mykofenolová analogy a deriváty MeSH
- ligace MeSH
- mediátory zánětu krev MeSH
- methylprednisolon farmakologie MeSH
- miniaturní prasata MeSH
- modely nemocí na zvířatech MeSH
- noradrenalin farmakologie MeSH
- prasata MeSH
- punkce MeSH
- sepse krev farmakoterapie imunologie mikrobiologie patofyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: The diagnosis of sepsis is difficult in immunocompromised patients owing to their modified response to infection. Our experiment in minipigs was designed to compare responses to sepsis between experimental groups of septic minipigs with and without immunosuppression. METHODS: Minipigs with identical baseline parameters were randomized into 3 groups: Sepsis (n = 10); immunosuppression (n = 11), including cyclosporine, methylprednisolone, and mycophenolate mofetil treatment before surgery, and a sham group (n = 6). Sepsis was induced by cecal ligation and puncture (CLP). We recorded selected clinical and laboratory parameters up to 24 hours postoperatively. RESULTS: All CLP animals developed septic shock with a febrile response, tachycardia, and hypotension requiring noradrenaline administration. The hemodynamic responses to sepsis in septic groups with and without immunosuppression were similar. Noradrenaline infusion was started on average later in the immunosuppression than in the group without immunosuppression; however, the difference was not significant. The kinetics of the plasma levels of most selected cytokines and C-reactive protein were similar in both septic groups. At 10 hours after surgery, the immunosuppression group showed significantly lower interleukin (IL)-6 levels compared with the sepsis group. At 19, 22, and 25 hours after surgery immunosuppressed animals displayed significantly greater increases in IL-10 levels compared with the cohort without immunosuppression. CONCLUSIONS: CLP is a simple, reproducible model of sepsis in minipigs. All CLP animals developed sepsis within 24 hours on average. Significant differences in IL-6 and IL-10 plasma levels were recorded between septic animals with versus without immunosuppression.
Department of Nephrology Institute for Clinical and Experimental Medicine Prague Czech Republic
Institute of Microbiology Academy of Sciences of the Czech Republic Czech Republic
Transplant Surgery Department Institute for Clinical and Experimental Medicine Prague Czech Republic
Citace poskytuje Crossref.org
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- $a The effect of immunosuppression on manifestations of sepsis in an animal model of cecal ligation and puncture / $c E. Kieslichova, M. Rocen, D. Merta, M. Kudla, I. Splichal, J. Cap, O. Viklicky, R. Gürlich
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- $a OBJECTIVE: The diagnosis of sepsis is difficult in immunocompromised patients owing to their modified response to infection. Our experiment in minipigs was designed to compare responses to sepsis between experimental groups of septic minipigs with and without immunosuppression. METHODS: Minipigs with identical baseline parameters were randomized into 3 groups: Sepsis (n = 10); immunosuppression (n = 11), including cyclosporine, methylprednisolone, and mycophenolate mofetil treatment before surgery, and a sham group (n = 6). Sepsis was induced by cecal ligation and puncture (CLP). We recorded selected clinical and laboratory parameters up to 24 hours postoperatively. RESULTS: All CLP animals developed septic shock with a febrile response, tachycardia, and hypotension requiring noradrenaline administration. The hemodynamic responses to sepsis in septic groups with and without immunosuppression were similar. Noradrenaline infusion was started on average later in the immunosuppression than in the group without immunosuppression; however, the difference was not significant. The kinetics of the plasma levels of most selected cytokines and C-reactive protein were similar in both septic groups. At 10 hours after surgery, the immunosuppression group showed significantly lower interleukin (IL)-6 levels compared with the sepsis group. At 19, 22, and 25 hours after surgery immunosuppressed animals displayed significantly greater increases in IL-10 levels compared with the cohort without immunosuppression. CONCLUSIONS: CLP is a simple, reproducible model of sepsis in minipigs. All CLP animals developed sepsis within 24 hours on average. Significant differences in IL-6 and IL-10 plasma levels were recorded between septic animals with versus without immunosuppression.
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- $a Rocen, M. $u Department of Anesthesiology and Intensive Care, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
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