-
Something wrong with this record ?
Collagen changes in the cochlea of aged Fischer 344 rats
D Buckiova, J Popelar, J Syka
Language English Country England, Great Britain
Document type Research Support, Non-U.S. Gov't
Grant support
NR8113
MZ0
CEP Register
- MeSH
- Azo Compounds analysis MeSH
- Coloring Agents analysis MeSH
- Organ of Corti chemistry MeSH
- Eosine Yellowish-(YS) analysis MeSH
- Fluorescent Dyes analysis MeSH
- Hematoxylin analysis MeSH
- Immunohistochemistry methods MeSH
- Cochlea * physiology chemistry MeSH
- Collagen Type II analysis MeSH
- Collagen * analysis MeSH
- Rats MeSH
- Tectorial Membrane chemistry MeSH
- Methyl Green analysis MeSH
- Rats, Inbred F344 MeSH
- Aging * physiology MeSH
- Stria Vascularis chemistry MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Hearing function in the Fischer 344 (F344) albino inbred strain of rats deteriorates with aging faster than in other strains, in spite of the small hair cell loss in old F344 animals [Popelar, J., Groh, D., Pelanova, J., Canlon, B., Syka, J., 2005. Age-related changes in cochlear and brainstem auditory function. Neurobiol. Aging, in press.]. This study was aimed at elucidating the structural changes in the inner ear of this rat strain during aging. Cochlear histopathology was examined in 20-24-month-old F344 rats and compared with that of young F344 rats (4 months) and of old rats of the Long-Evans (LE) strain. Hematoxylin/eosin staining in aged F344 rats showed degenerative changes in the organ of Corti, consisting of a damaged layer of marginal cells, reduced vascularization of the stria vascularis and a distorted tectorial membrane detached from the organ of Corti. Age-related changes in collagen distribution were observed with Masson's trichrome staining in the spiral ligament of old F344 rats. The results of immunohistochemical staining for type II collagen revealed a marked decrease in collagen fibers in the area connecting the spiral ligament and stria vascularis and a decrease in area IV fibrocytes in old F344 but not in LE rats. These findings may contribute to an explanation of the substantial hearing loss found in old F344 rats.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13032821
- 003
- CZ-PrNML
- 005
- 20131101092221.0
- 007
- ta
- 008
- 131010s2006 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.exger.2005.11.010 $2 doi
- 035 __
- $a (PubMed)16427232
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Buckiová, Daniela $7 xx0063935 $u Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. bucki@biomed.cas.cz
- 245 10
- $a Collagen changes in the cochlea of aged Fischer 344 rats / $c D Buckiova, J Popelar, J Syka
- 520 9_
- $a Hearing function in the Fischer 344 (F344) albino inbred strain of rats deteriorates with aging faster than in other strains, in spite of the small hair cell loss in old F344 animals [Popelar, J., Groh, D., Pelanova, J., Canlon, B., Syka, J., 2005. Age-related changes in cochlear and brainstem auditory function. Neurobiol. Aging, in press.]. This study was aimed at elucidating the structural changes in the inner ear of this rat strain during aging. Cochlear histopathology was examined in 20-24-month-old F344 rats and compared with that of young F344 rats (4 months) and of old rats of the Long-Evans (LE) strain. Hematoxylin/eosin staining in aged F344 rats showed degenerative changes in the organ of Corti, consisting of a damaged layer of marginal cells, reduced vascularization of the stria vascularis and a distorted tectorial membrane detached from the organ of Corti. Age-related changes in collagen distribution were observed with Masson's trichrome staining in the spiral ligament of old F344 rats. The results of immunohistochemical staining for type II collagen revealed a marked decrease in collagen fibers in the area connecting the spiral ligament and stria vascularis and a decrease in area IV fibrocytes in old F344 but not in LE rats. These findings may contribute to an explanation of the substantial hearing loss found in old F344 rats.
- 590 __
- $a bohemika - dle Pubmed
- 650 12
- $a stárnutí $x fyziologie $7 D000375
- 650 02
- $a zvířata $7 D000818
- 650 02
- $a azosloučeniny $x analýza $7 D001391
- 650 12
- $a kochlea $x fyziologie $x chemie $7 D003051
- 650 12
- $a kolagen $x analýza $7 D003094
- 650 02
- $a kolagen typ II $x analýza $7 D024043
- 650 02
- $a barvicí látky $x analýza $7 D004396
- 650 02
- $a eosin $x analýza $7 D004801
- 650 02
- $a fluorescenční barviva $x analýza $7 D005456
- 650 02
- $a hematoxylin $x analýza $7 D006416
- 650 02
- $a imunohistochemie $x metody $7 D007150
- 650 02
- $a methylová zeleň $x analýza $7 D008739
- 650 02
- $a Cortiho orgán $x chemie $7 D009925
- 650 02
- $a krysa rodu Rattus $7 D051381
- 650 02
- $a potkani inbrední F344 $7 D011916
- 650 02
- $a stria vascularis $x chemie $7 D013316
- 650 02
- $a membrana tectoria $x chemie $7 D013680
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Popelář, Jiří, $d 1949- $7 xx0085571 $u Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
- 700 1_
- $a Syka, Josef, $d 1940- $7 jk01122179 $u Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
- 773 0_
- $t Experimental Gerontology $x 0531-5565 $g Roč. 41, č. 3 (2006), s. 296-302 $p Exp Gerontol $w MED00001745
- 910 __
- $a ABA008 $b A 3795 $y 3 $z 0
- 990 __
- $a 20131010145855 $b ABA008
- 991 __
- $a 20131101092734 $b ABA008
- 999 __
- $a ok $b bmc $g 996988 $s 831272
- BAS __
- $a 3
- BMC __
- $a 2006 $b 41 $c 3 $d 296-302 $x MED00001745 $i 0531-5565 $m Experimental gerontology
- GRA __
- $a NR8113 $p MZ0
- LZP __
- $a NLK 2013-10/lpbo
