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Štúdium molten globulárneho stavu cytochrómu C pomocou viskozimetrie
[Study of the molten globule state of cytochrome C by Viscometry]
M. Stupák, M. Antalík
Language Slovak Country Czech Republic
- MeSH
- Circular Dichroism * methods instrumentation utilization MeSH
- Cytochromes c * analysis ultrastructure MeSH
- Protein Conformation * MeSH
- Proteins chemistry MeSH
- Spectrum Analysis methods instrumentation utilization MeSH
- Viscosity * MeSH
Combination of viscometry and CD spectrometry made it possible to suggest the existence of a new conformational state of cytochrome c at high temperatures and ionic strengths. The molten globule state of cytochrome c was found at acidic pH in the presence of high concentrations of a salt at 20 °C. A low-cooperative conformational change of the cytochrome c to the unfolded state was observed with increasing temperature. Viscometry is a suitable tool for studies of similar processes in biomacromolecules.
Department of Biochemistry Faculty of Natural Science P J Šafárik University Košice
Department of Medicinal and Clinical Biochemistry and Labmed Co Faculty of Medicine Košice
Depatment of Biophysics Institute of Experimental Phy¬sics of Slovak Acadamy of Sciences Košice
Study of the molten globule state of cytochrome C by Viscometry
Literatura
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- $a Combination of viscometry and CD spectrometry made it possible to suggest the existence of a new conformational state of cytochrome c at high temperatures and ionic strengths. The molten globule state of cytochrome c was found at acidic pH in the presence of high concentrations of a salt at 20 °C. A low-cooperative conformational change of the cytochrome c to the unfolded state was observed with increasing temperature. Viscometry is a suitable tool for studies of similar processes in biomacromolecules.
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