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Fibroblast growth factor-21 is expressed in neonatal and pheochromocytoma-induced adult human brown adipose tissue
E. Hondares, JM. Gallego-Escuredo, P. Flachs, A. Frontini, R. Cereijo, A. Goday, J. Perugini, P. Kopecky, M. Giralt, S. Cinti, J. Kopecky, F. Villarroya,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adipose Tissue, White metabolism MeSH
- Adult MeSH
- Pheochromocytoma genetics metabolism MeSH
- Fibroblast Growth Factors genetics metabolism MeSH
- Adipose Tissue, Brown metabolism MeSH
- Adipocytes, Brown metabolism MeSH
- Ion Channels genetics metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- 4-1BB Ligand genetics metabolism MeSH
- Mitochondrial Proteins genetics metabolism MeSH
- Adrenal Gland Neoplasms genetics metabolism MeSH
- Infant, Newborn MeSH
- Aged MeSH
- Transcription Factors genetics metabolism MeSH
- Transcriptome genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Infant, Newborn MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
OBJECTIVE: In rodents, brown (BAT) and white (WAT) adipose tissues are targets and expression sites for fibroblast growth factor-21 (FGF21). In contrast, human WAT expresses negligible levels of FGF21. We examined FGF21 expression in human BAT samples, including the induced BAT found in adult patients with pheochromocytoma, and interscapular and visceral BAT from newborns. METHODS: The expression of FGF21 and uncoupling protein-1 (UCP1, a brown adipocyte marker), was determined by quantitative real-time-PCR and immunoblotting. The transcript levels of marker genes for developmentally-programmed BAT (zinc-finger-protein of the cerebellum-1, ZIC1) and inducible-BAT (cluster of differentiation-137, CD137) were also determined. RESULTS: FGF21 and UCP1 are significantly expressed in visceral adipose tissue from pheochromocytoma patients, but not in visceral fat from healthy individuals. In neonates, FGF21 and UCP1 are both expressed in visceral and interscapular fat, and their expression levels show a significant positive correlation. Marker gene expression profiles suggest that inducible BAT is present in visceral fat from pheochromocytoma patients and neonates, whereas developmentally-programmed BAT is present in neonatal interscapular fat. CONCLUSIONS: Human BAT, but not WAT, expresses FGF21. The expression of FGF21 is especially high in inducible, also called beige/brite, neonatal BAT, but it is also found in the interscapular, developmentally-programmed, BAT of neonates.
CIBER Fisiopatologia de la Obesidad y Nutricion Instituto de Salud Carlos 3 Spain
Department of Endocrinology and Nutrition Hospital del Mar Barcelona Spain
References provided by Crossref.org
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- $a Hondares, Elayne $u Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, Barcelona, Spain; CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Spain.
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- $a Fibroblast growth factor-21 is expressed in neonatal and pheochromocytoma-induced adult human brown adipose tissue / $c E. Hondares, JM. Gallego-Escuredo, P. Flachs, A. Frontini, R. Cereijo, A. Goday, J. Perugini, P. Kopecky, M. Giralt, S. Cinti, J. Kopecky, F. Villarroya,
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- $a OBJECTIVE: In rodents, brown (BAT) and white (WAT) adipose tissues are targets and expression sites for fibroblast growth factor-21 (FGF21). In contrast, human WAT expresses negligible levels of FGF21. We examined FGF21 expression in human BAT samples, including the induced BAT found in adult patients with pheochromocytoma, and interscapular and visceral BAT from newborns. METHODS: The expression of FGF21 and uncoupling protein-1 (UCP1, a brown adipocyte marker), was determined by quantitative real-time-PCR and immunoblotting. The transcript levels of marker genes for developmentally-programmed BAT (zinc-finger-protein of the cerebellum-1, ZIC1) and inducible-BAT (cluster of differentiation-137, CD137) were also determined. RESULTS: FGF21 and UCP1 are significantly expressed in visceral adipose tissue from pheochromocytoma patients, but not in visceral fat from healthy individuals. In neonates, FGF21 and UCP1 are both expressed in visceral and interscapular fat, and their expression levels show a significant positive correlation. Marker gene expression profiles suggest that inducible BAT is present in visceral fat from pheochromocytoma patients and neonates, whereas developmentally-programmed BAT is present in neonatal interscapular fat. CONCLUSIONS: Human BAT, but not WAT, expresses FGF21. The expression of FGF21 is especially high in inducible, also called beige/brite, neonatal BAT, but it is also found in the interscapular, developmentally-programmed, BAT of neonates.
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- $a Gallego-Escuredo, José M $u Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, Barcelona, Spain; CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Spain.
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- $a Flachs, Pavel $u Department of Adipose Tissue Biology, Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
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- $a Cereijo, Ruben $u Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, Barcelona, Spain; CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Spain.
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- $a Perugini, Jessica $u Department of Experimental and Clinical Medicine, Center for the Study of Obesity-United Hospitals University of Ancona (Politecnica delle Marche), Ancona, Italy.
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- $a Giralt, Marta $u Department of Biochemistry and Molecular Biology, and Institute of Biomedicine (IBUB), University of Barcelona, Barcelona, Spain; CIBER Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Spain.
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- $a Cinti, Saverio $u Department of Experimental and Clinical Medicine, Center for the Study of Obesity-United Hospitals University of Ancona (Politecnica delle Marche), Ancona, Italy.
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- $a Kopecky, Jan $u Department of Adipose Tissue Biology, Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
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