• Je něco špatně v tomto záznamu ?

Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants

A. Ogrocká, P. Polanská, E. Majerová, Z. Janeba, J. Fajkus, M. Fojtová,

. 2014 ; 42 (5) : 2919-31.

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc14063817

Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14063817
003      
CZ-PrNML
005      
20140707113036.0
007      
ta
008      
140704s2014 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/nar/gkt1285 $2 doi
035    __
$a (PubMed)24334955
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Ogrocká, Anna $u Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, 62500 Brno, Czech Republic, Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic v.v.i., Flemingovo nám. 2, 166 10, Prague, Czech Republic and Institute of Biophysics, Academy of Sciences of the Czech Republic v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
245    10
$a Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants / $c A. Ogrocká, P. Polanská, E. Majerová, Z. Janeba, J. Fajkus, M. Fojtová,
520    9_
$a Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems.
650    _2
$a Arabidopsis $x enzymologie $x genetika $x metabolismus $7 D017360
650    _2
$a cytosin $x metabolismus $7 D003596
650    12
$a metylace DNA $7 D019175
650    _2
$a rostliny $x genetika $x metabolismus $7 D010944
650    _2
$a repetitivní sekvence nukleových kyselin $7 D012091
650    _2
$a telomerasa $x metabolismus $7 D019098
650    _2
$a telomery $x chemie $x metabolismus $7 D016615
650    _2
$a homeostáza telomer $7 D059505
650    12
$a zkracování telomer $7 D059506
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Polanská, Pavla
700    1_
$a Majerová, Eva
700    1_
$a Janeba, Zlatko
700    1_
$a Fajkus, Jirí
700    1_
$a Fojtová, Miloslava
773    0_
$w MED00003554 $t Nucleic acids research $x 1362-4962 $g Roč. 42, č. 5 (2014), s. 2919-31
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24334955 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20140704 $b ABA008
991    __
$a 20140707113325 $b ABA008
999    __
$a ok $b bmc $g 1031301 $s 862549
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 42 $c 5 $d 2919-31 $i 1362-4962 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a Pubmed-20140704

Najít záznam

Citační ukazatele

    Možnosti archivace