• Something wrong with this record ?

Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery

F. Cvrčková, M. Hála,

. 2014 ; 1080 (-) : 267-82.

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc14063885

The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14063885
003      
CZ-PrNML
005      
20140707113447.0
007      
ta
008      
140704s2014 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/978-1-62703-643-6_23 $2 doi
035    __
$a (PubMed)24132437
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Cvrčková, Fatima $u Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
245    10
$a Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery / $c F. Cvrčková, M. Hála,
520    9_
$a The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.
650    _2
$a exprese genu $7 D015870
650    _2
$a genová knihovna $7 D015723
650    12
$a rostlinné geny $7 D017343
650    _2
$a testy genetické komplementace $7 D005816
650    _2
$a fenotyp $7 D010641
650    _2
$a rostlinné buňky $x metabolismus $7 D059828
650    _2
$a vývoj rostlin $x fyziologie $7 D063245
650    _2
$a plazmidy $x genetika $7 D010957
650    _2
$a Saccharomycetales $x genetika $7 D004718
650    _2
$a transformace genetická $7 D014170
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hála, Michal
773    0_
$w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1080, č. - (2014), s. 267-82
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24132437 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20140704 $b ABA008
991    __
$a 20140707113736 $b ABA008
999    __
$a ok $b bmc $g 1031369 $s 862617
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 1080 $c - $d 267-82 $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
LZP    __
$a Pubmed-20140704

Find record

Citation metrics

Archiving options