-
Something wrong with this record ?
Lichen secondary metabolites as DNA-interacting agents
J. Plsíkova, J. Stepankova, J. Kasparkova, V. Brabec, M. Backor, M. Kozurkova,
Toxicology in vitro.
2014 ;
28 (2) :
182-6.
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/bmc14074479
- MeSH
- Benzoates pharmacology MeSH
- Benzofurans pharmacology MeSH
- Circular Dichroism MeSH
- DNA drug effects metabolism MeSH
- Emodin analogs & derivatives pharmacology MeSH
- Hydroxybenzoates pharmacology MeSH
- Topoisomerase I Inhibitors pharmacology MeSH
- Topoisomerase II Inhibitors pharmacology MeSH
- Intercalating Agents pharmacology MeSH
- Kinetics MeSH
- Nucleic Acid Conformation MeSH
- Humans MeSH
- Lichens chemistry metabolism MeSH
- Cattle MeSH
- Spectrophotometry, Ultraviolet MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc14074479
- 003
- CZ-PrNML
- 005
- 20141008104044.0
- 007
- ta
- 008
- 141006s2014 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.tiv.2013.11.003 $2 doi
- 035 __
- $a (PubMed)24269500
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Plsíkova, J $u Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia.
- 245 10
- $a Lichen secondary metabolites as DNA-interacting agents / $c J. Plsíkova, J. Stepankova, J. Kasparkova, V. Brabec, M. Backor, M. Kozurkova,
- 520 9_
- $a A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a benzoáty $x farmakologie $7 D001565
- 650 _2
- $a benzofurany $x farmakologie $7 D001572
- 650 _2
- $a skot $7 D002417
- 650 _2
- $a cirkulární dichroismus $7 D002942
- 650 _2
- $a DNA $x účinky léků $x metabolismus $7 D004247
- 650 _2
- $a emodin $x analogy a deriváty $x farmakologie $7 D004642
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a hydroxybenzoáty $x farmakologie $7 D062385
- 650 _2
- $a interkalátory $x farmakologie $7 D007364
- 650 _2
- $a kinetika $7 D007700
- 650 _2
- $a lišejníky $x chemie $x metabolismus $7 D008011
- 650 _2
- $a konformace nukleové kyseliny $7 D009690
- 650 _2
- $a spektrofotometrie ultrafialová $7 D013056
- 650 _2
- $a inhibitory topoisomerasy I $x farmakologie $7 D059004
- 650 _2
- $a inhibitory topoisomerasy II $x farmakologie $7 D059005
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Stepankova, J $u Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
- 700 1_
- $a Kasparkova, J $u Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
- 700 1_
- $a Brabec, V $u Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
- 700 1_
- $a Backor, M $u Institute of Biology and Ecology, Department of Botany, Pavol Jozef Šafárik University in Košice, Slovakia.
- 700 1_
- $a Kozurkova, M $u Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia. Electronic address: maria.kozurkova@upjs.sk.
- 773 0_
- $w MED00004536 $t Toxicology in vitro : an international journal published in association with BIBRA $x 1879-3177 $g Roč. 28, č. 2 (2014), s. 182-6
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24269500 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20141006 $b ABA008
- 991 __
- $a 20141008104433 $b ABA008
- 999 __
- $a ok $b bmc $g 1042362 $s 873391
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 28 $c 2 $d 182-6 $i 1879-3177 $m Toxicology in vitro $n Toxicol In Vitro $x MED00004536
- LZP __
- $a Pubmed-20141006
