-
Something wrong with this record ?
Large volume sample stacking for rapid and sensitive determination of antidiabetic drug metformin in human urine and serum by capillary electrophoresis with contactless conductivity detection
P. Tůma,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Time Factors MeSH
- Diabetes Mellitus, Type 2 blood urine MeSH
- Electrophoresis, Capillary methods MeSH
- Hypoglycemic Agents blood urine MeSH
- Calibration MeSH
- Humans MeSH
- Metformin blood urine MeSH
- Reproducibility of Results MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14 cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35s and the LOD is 0.3 μM. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 μM and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1% for urine and 0.7% for serum; RSD for the peak areas equalled 1.4% for urine and 2.6% for serum. The developed CE technique was tested on performance of routine analyses of metformin in the urine and serum of patients suffering from type II diabetes mellitus.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15008070
- 003
- CZ-PrNML
- 005
- 20150306132956.0
- 007
- ta
- 008
- 150306s2014 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.chroma.2014.04.016 $2 doi
- 035 __
- $a (PubMed)24792694
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Tůma, Petr $u Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic. Electronic address: petr.tuma@lf3.cuni.cz.
- 245 10
- $a Large volume sample stacking for rapid and sensitive determination of antidiabetic drug metformin in human urine and serum by capillary electrophoresis with contactless conductivity detection / $c P. Tůma,
- 520 9_
- $a Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14 cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35s and the LOD is 0.3 μM. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 μM and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1% for urine and 0.7% for serum; RSD for the peak areas equalled 1.4% for urine and 2.6% for serum. The developed CE technique was tested on performance of routine analyses of metformin in the urine and serum of patients suffering from type II diabetes mellitus.
- 650 _2
- $a kalibrace $7 D002138
- 650 _2
- $a diabetes mellitus 2. typu $x krev $x moč $7 D003924
- 650 _2
- $a elektroforéza kapilární $x metody $7 D019075
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a hypoglykemika $x krev $x moč $7 D007004
- 650 _2
- $a metformin $x krev $x moč $7 D008687
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 650 _2
- $a časové faktory $7 D013997
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 773 0_
- $w MED00004962 $t Journal of chromatography. A $x 1873-3778 $g Roč. 1345, č. - (2014), s. 207-11
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24792694 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150306 $b ABA008
- 991 __
- $a 20150306133227 $b ABA008
- 999 __
- $a ok $b bmc $g 1065343 $s 890870
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 1345 $c - $d 207-11 $i 1873-3778 $m Journal of chromatography. A, Including electrophoresis and other separation methods $n J Chromatogr A $x MED00004962
- LZP __
- $a Pubmed-20150306