• Je něco špatně v tomto záznamu ?

Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

L. Krásný, E. Rohlová, H. Růžičková, J. Santrůček, R. Hynek, I. Hochel,

. 2014 ; 98 (-) : 105-13.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15008374

Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15008374
003      
CZ-PrNML
005      
20150327115145.0
007      
ta
008      
150306s2014 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.mimet.2014.01.008 $2 doi
035    __
$a (PubMed)24462807
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Krásný, Lukáš $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic; Institute of Microbiology, AS CR, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: krasnyl@vscht.cz.
245    10
$a Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis / $c L. Krásný, E. Rohlová, H. Růžičková, J. Santrůček, R. Hynek, I. Hochel,
520    9_
$a Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species.
650    _2
$a techniky typizace bakterií $x metody $7 D015373
650    _2
$a bakteriologické techniky $x metody $7 D001431
650    _2
$a Cronobacter $x izolace a purifikace $7 D059126
650    _2
$a Cronobacter sakazakii $x izolace a purifikace $7 D044083
650    _2
$a polymerázová řetězová reakce $x metody $7 D016133
650    _2
$a software $7 D012984
650    _2
$a spektrometrie hmotnostní - ionizace laserem za účasti matrice $x metody $7 D019032
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Rohlová, Eva $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
700    1_
$a Růžičková, Helena $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
700    1_
$a Santrůček, Jiří $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
700    1_
$a Hynek, Radovan $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
700    1_
$a Hochel, Igor $u Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
773    0_
$w MED00002803 $t Journal of microbiological methods $x 1872-8359 $g Roč. 98, č. - (2014), s. 105-13
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24462807 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150306 $b ABA008
991    __
$a 20150327115508 $b ABA008
999    __
$a ok $b bmc $g 1065647 $s 891174
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 98 $c - $d 105-13 $i 1872-8359 $m Journal of microbiological methods $n J Microbiol Methods $x MED00002803
LZP    __
$a Pubmed-20150306

Najít záznam

Citační ukazatele

Možnosti archivace