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Analysis of intracellular nucleotides by capillary electrophoresis-mass spectrometry
D Friedecky, P Bednar, M Prochazka, T Adam
Jazyk angličtina Země Spojené státy americké
Typ dokumentu práce podpořená grantem
Grantová podpora
NR8578
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
NLK
Medline Complete (EBSCOhost)
od 2001-01-01 do Před 1 rokem
- MeSH
- aminoimidazolkarboxamid analogy a deriváty farmakologie chemie MeSH
- elektroforéza kapilární metody přístrojové vybavení MeSH
- erytrocyty * cytologie metabolismus MeSH
- hmotnostní spektrometrie * metody MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- nukleotidy analýza chemie izolace a purifikace MeSH
- puriny chemie MeSH
- ribonukleosidy farmakologie chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.
Department of analytical chemistry Palacký University Olomouc Czech Republic
Department of Obsterics and ynecology University Hospital Olomouc Czech Republic
Citace poskytuje Crossref.org
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- $a A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.
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