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Analysis of intracellular nucleotides by capillary electrophoresis-mass spectrometry

D Friedecky, P Bednar, M Prochazka, T Adam

. 2006 ; 25 (9-11) : 1233-1236.

Language English Country United States

Document type Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc15011165

Grant support
NR8578 MZ0 CEP Register

A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.

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$a A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.
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