A nanopore-based devices are extremely sensitive analytical techniques, which uses the electrophoretic translocation of molecules in solution through a nano-scale pores. The nanopores, which mimic the functions of natural ion channels, seems to be the promissing tool for future fast and low-cost DNA sequencing. However, some difficulties in generating usable sequence data have to be solved. In this article the nanopores were reviewed. In the first part the development of nanopore technique was described and the ubiquitous presence of nanopores in living cells was highlighted. Next, the most important part of the principles of nanopore analysis was described, and the knowledges about biological and solid-state nanopores were summarized. Also the pros and cons of both kinds of nanopores and different approaches designed to circumvent the issues were mentioned.

Středoevropský technologický institut Vysoké učení technické v Brně

Ústav chemie a biochemie Agronomická fakulta Mendelova univerzita v Brně

Nanopores as a modern tool for DNA sequencing

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