• Je něco špatně v tomto záznamu ?

Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis

AC. Lindner, D. Lang, M. Seifert, K. Podlešáková, O. Novák, M. Strnad, R. Reski, K. von Schwartzenberg,

. 2014 ; 65 (9) : 2533-43.

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15014434

The moss Physcomitrella patens is part of an early divergent clade of land plants utilizing the plant hormone cytokinin for growth control. The rate-limiting step of cytokinin biosynthesis is mediated by isopentenyltransferases (IPTs), found in land plants either as adenylate-IPTs or as tRNA-IPTs. Although a dominant part of cytokinins in flowering plants are synthesized by adenylate-IPTs, the Physcomitrella genome only encodes homologues of tRNA-IPTs. This study therefore looked into the question of whether cytokinins in moss derive from tRNA exclusively. Targeted gene knockout of ipt1 (d|ipt1) along with localization studies revealed that the chloroplast-bound IPT1 was almost exclusively responsible for the A37 prenylation of tRNA in Physcomitrella. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based cytokinin profiling demonstrated that the total amount of all free cytokinins in tissue was almost unaffected. However, the knockout plants showed increased levels of the N (6) -isopentenyladenine (iP)- and trans-zeatin (tZ)-type cytokinins, considered to provide active forms, while cis-zeatin (cZ)-type cytokinins were reduced. The data provide evidence for an additional and unexpected tRNA-independent cytokinin biosynthetic pathway in moss. Comprehensive phylogenetic analysis indicates a diversification of tRNA-IPT-like genes in bryophytes probably related to additional functions.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15014434
003      
CZ-PrNML
005      
20150427124438.0
007      
ta
008      
150420s2014 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/jxb/eru142 $2 doi
035    __
$a (PubMed)24692654
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Lindner, Ann-Cathrin $u University of Hamburg, Biocenter Klein Flottbek, Ohnhorststraße 18, D-22609 Hamburg, Germany.
245    10
$a Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis / $c AC. Lindner, D. Lang, M. Seifert, K. Podlešáková, O. Novák, M. Strnad, R. Reski, K. von Schwartzenberg,
520    9_
$a The moss Physcomitrella patens is part of an early divergent clade of land plants utilizing the plant hormone cytokinin for growth control. The rate-limiting step of cytokinin biosynthesis is mediated by isopentenyltransferases (IPTs), found in land plants either as adenylate-IPTs or as tRNA-IPTs. Although a dominant part of cytokinins in flowering plants are synthesized by adenylate-IPTs, the Physcomitrella genome only encodes homologues of tRNA-IPTs. This study therefore looked into the question of whether cytokinins in moss derive from tRNA exclusively. Targeted gene knockout of ipt1 (d|ipt1) along with localization studies revealed that the chloroplast-bound IPT1 was almost exclusively responsible for the A37 prenylation of tRNA in Physcomitrella. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based cytokinin profiling demonstrated that the total amount of all free cytokinins in tissue was almost unaffected. However, the knockout plants showed increased levels of the N (6) -isopentenyladenine (iP)- and trans-zeatin (tZ)-type cytokinins, considered to provide active forms, while cis-zeatin (cZ)-type cytokinins were reduced. The data provide evidence for an additional and unexpected tRNA-independent cytokinin biosynthetic pathway in moss. Comprehensive phylogenetic analysis indicates a diversification of tRNA-IPT-like genes in bryophytes probably related to additional functions.
650    _2
$a alkyltransferasy a aryltransferasy $x genetika $x metabolismus $7 D019883
650    12
$a biologická evoluce $7 D005075
650    _2
$a mechy $x klasifikace $x enzymologie $x genetika $7 D019068
650    _2
$a chloroplasty $x enzymologie $x genetika $7 D002736
650    _2
$a cytokininy $x biosyntéza $7 D003583
650    _2
$a genový knockout $7 D055786
650    _2
$a molekulární sekvence - údaje $7 D008969
650    12
$a fylogeneze $7 D010802
650    _2
$a rostlinné proteiny $x genetika $x metabolismus $7 D010940
650    _2
$a rostliny $x klasifikace $x enzymologie $x genetika $7 D010944
650    _2
$a RNA transferová $x genetika $x metabolismus $7 D012343
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Lang, Daniel $u University of Freiburg, Faculty of Biology, Plant Biotechnology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
700    1_
$a Seifert, Maike $u University of Hamburg, Biocenter Klein Flottbek, Ohnhorststraße 18, D-22609 Hamburg, Germany.
700    1_
$a Podlešáková, Kateřina $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR and Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic Palacký University, Department of Biochemistry, Šlechtitelů 11, 78371 Olomouc, Czech Republic.
700    1_
$a Novák, Ondřej $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR and Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic.
700    1_
$a Strnad, Miroslav $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR and Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic.
700    1_
$a Reski, Ralf $u University of Freiburg, Faculty of Biology, Plant Biotechnology, Schaenzlestr. 1, D-79104 Freiburg, Germany FRIAS-Freiburg Institute for Advanced Studies, Freiburg, Germany BIOSS-Centre for Biological Signalling Studies, Freiburg, Germany.
700    1_
$a von Schwartzenberg, Klaus $u University of Hamburg, Biocenter Klein Flottbek, Ohnhorststraße 18, D-22609 Hamburg, Germany klaus.von.schwartzenberg@uni-hamburg.de.
773    0_
$w MED00006559 $t Journal of experimental botany $x 1460-2431 $g Roč. 65, č. 9 (2014), s. 2533-43
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24692654 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150420 $b ABA008
991    __
$a 20150427124741 $b ABA008
999    __
$a ok $b bmc $g 1072015 $s 897312
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 65 $c 9 $d 2533-43 $i 1460-2431 $m Journal of Experimental Botany $n J Exp Bot $x MED00006559
LZP    __
$a Pubmed-20150420

Najít záznam

Citační ukazatele

    Možnosti archivace