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Enterobacter cloacae with a novel variant of ACT AmpC beta-lactamase originating from glaucous gull (Larus hyperboreus) in Svalbard
I. Literak, I. Manga, K. Wojczulanis-Jakubas, M. Chroma, I. Jamborova, H. Dobiasova, MH. Sedlakova, A. Cizek,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Drug Resistance, Bacterial genetics MeSH
- Bacterial Proteins genetics metabolism MeSH
- beta-Lactamases genetics metabolism MeSH
- Charadriiformes microbiology MeSH
- DNA Primers genetics MeSH
- Enterobacter cloacae enzymology genetics MeSH
- Escherichia coli genetics MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Cloaca microbiology MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction veterinary MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA veterinary MeSH
- Amino Acid Substitution genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Norway MeSH
- Svalbard MeSH
We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146 bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.
CEITEC VFU Veterinary and Pharmaceutical University Brno Brno Czech Republic
Department of Vertebrate Ecology and Zoology University of Gdansk Gdansk Poland
References provided by Crossref.org
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