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Enterobacter cloacae with a novel variant of ACT AmpC beta-lactamase originating from glaucous gull (Larus hyperboreus) in Svalbard
I. Literak, I. Manga, K. Wojczulanis-Jakubas, M. Chroma, I. Jamborova, H. Dobiasova, MH. Sedlakova, A. Cizek,
Veterinary microbiology.
2014 ;
171 (3-4) :
432-5.
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
Perzistentní odkaz
https://www.medvik.cz/link/bmc15014475
- MeSH
- bakteriální léková rezistence genetika MeSH
- bakteriální proteiny genetika metabolismus MeSH
- beta-laktamasy genetika metabolismus MeSH
- Charadriiformes mikrobiologie MeSH
- DNA primery genetika MeSH
- Enterobacter cloacae enzymologie genetika MeSH
- Escherichia coli genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- kloaka mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce veterinární MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA veterinární MeSH
- substituce aminokyselin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Norsko MeSH
- Svalbard MeSH
We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146 bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.
CEITEC VFU Veterinary and Pharmaceutical University Brno Brno Czech Republic
Department of Vertebrate Ecology and Zoology University of Gdansk Gdansk Poland
Citace poskytuje Crossref.org
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