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Peptidases of Trichobilharzia regenti (Schistosomatidae) and its molluscan host Radix peregra S. Lat. (Lymnaeidae): construction and screening of cDNA library from intramolluscan stages of the parasite
K. Dolecková, M. Kasný, L. Mikes, F. Mutapi, C. Stack, AP. Mountford, P. Horák,
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1966
ProQuest Central
od 2004-01-01 do Před 3 měsíci
Health & Medicine (ProQuest)
od 2004-01-01 do Před 3 měsíci
Public Health Database (ProQuest)
od 2004-01-01 do Před 3 měsíci
ROAD: Directory of Open Access Scholarly Resources
od 1982
- MeSH
- cysteinové endopeptidasy genetika MeSH
- genová knihovna MeSH
- hlemýždi parazitologie MeSH
- kathepsin B genetika MeSH
- kathepsin L MeSH
- kathepsiny genetika MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce MeSH
- proteasy genetika MeSH
- proteiny červů genetika MeSH
- Schistosomatidae enzymologie genetika růst a vývoj MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie aminokyselin MeSH
- stadia vývoje MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Trichobilharzia regenti is a neurotropic bird schistosome,causing cercarial dermatitis in humans. In this study, ZAP cDNA expression library from Radix peregra s. lat. hepatopancreases containing intramolluscan stages of T. regenti was constructed and screened using PCR with specific and degenerate primers, designed according to previously described serine and cysteine peptidases of other parasite species. Full-length sequences of cathepsins B1 and L, and two serine peptidases, named RpSP1 and RpSP2, were obtained. The protein-protein BLAST analysis and parallel control reactions with template from hepatopancreases of T. regenti non-infected snails revealed that only cathepsin B1 was of parasite origin. The remaining sequences were derived from the snail intermediate host, which implies that the initial source of parasite mRNA was contaminated by snail tissue. Regardless of this contamination, the cDNA library remains an excellent molecular tool for detection and identification of bioactive molecules in T. regenti cercariae.
Citace poskytuje Crossref.org
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