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Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry
D. Moravcová, M. Haapala, J. Planeta, T. Hyötyläinen, R. Kostiainen, SK. Wiedmer,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Spectrometry, Mass, Electrospray Ionization instrumentation methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Nucleosides chemistry isolation & purification MeSH
- Nucleotides chemistry isolation & purification MeSH
- Silicon Dioxide chemistry MeSH
- Surface Properties MeSH
- Reproducibility of Results MeSH
- Tandem Mass Spectrometry instrumentation methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm × 0.1mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methacryloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min.
Department of Chemistry P O Box 55 00014 University of Helsinki Finland
Faculty of Pharmacy P O Box 56 00014 University of Helsinki Finland
Institute of Analytical Chemistry of the ASCR v v i Veveří 97 60200 Brno Czech Republic
Steno Diabetes Center Niels Steensens Vej 2 2820 Gentofte Denmark
References provided by Crossref.org
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