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Absence of maternal cell contamination in mesenchymal stromal cell cultures derived from equine umbilical cord tissue
I. Vackova, V. Czernekova, M. Tomanek, J. Navratil, T. Mosko, Z. Novakova,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- koně * MeSH
- kultivované buňky * MeSH
- mezenchymální kmenové buňky * MeSH
- mikrosatelitní repetice MeSH
- pupečník cytologie MeSH
- těhotenství MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin.
Department of Animal Husbandry Czech University of Life Sciences Prague Czech Republic
Department of Biology of Reproduction Institute of Animal Science Prague Czech Republic
Department of Molecular Genetics Institute of Animal Science Prague Czech Republic
Citace poskytuje Crossref.org
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- $a Vackova, I $u Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. Electronic address: ivackova@seznam.cz.
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- $a This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin.
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- $a Czernekova, V $u Department of Molecular Genetics, Institute of Animal Science, Prague, Czech Republic.
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- $a Navratil, J $u Department of Animal Husbandry, Czech University of Life Sciences, Prague, Czech Republic.
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- $a Mosko, T $u Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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- $a Novakova, Z $u Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic.
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