-
Something wrong with this record ?
Assay of urinary 8-hydroxy-2'-deoxyguanosine by capillary electrophoresis with spectrophotometric detection using a high-sensitivity detection cell and solid-phase extraction
K. Petrů, J. Siroká, L. Bydžovská, L. Krčmová, M. Polášek,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Deoxyguanosine analogs & derivatives chemistry urine MeSH
- Electrophoresis, Capillary methods MeSH
- Solid Phase Extraction methods MeSH
- Humans MeSH
- Limit of Detection MeSH
- Linear Models MeSH
- Reproducibility of Results MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A sensitive capillary electrophoretic method featuring spectrophotometric detection using a commercial Z-cell was devised for the assay of 8-hydroxy-2'-deoxyguanosine (8OHdG) in human urine. Solid-phase extraction (SPE) based on hydrophilic-lipophilic-balanced RP sorbent was utilized for urine sample pretreatment and analyte preconcentration. The separation was carried out in conventional fused-silica capillaries employing a Z-cell with hydrodynamic sample injection (at 50 mbar for 12 s). The BGE (pH* 9.2, adjusted with 1 M NaOH) contained 0.15 M boric acid and 10% v/v ACN. The detection wavelength was 282 nm. The calibration curve for 8OHdG (measured in spiked urine) was linear in the range 10-1000 ng/mL; R(2) = 0.9993. The LOD was 3 ng/mL (11 nmol/L) of 8OHdG. Determination of the 8OHdG urinary levels was possible even in healthy individuals.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15023444
- 003
- CZ-PrNML
- 005
- 20161227111505.0
- 007
- ta
- 008
- 150709s2014 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/elps.201300605 $2 doi
- 035 __
- $a (PubMed)24789757
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Petrů, Klára $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského, Hradec Králové, Czech Republic.
- 245 10
- $a Assay of urinary 8-hydroxy-2'-deoxyguanosine by capillary electrophoresis with spectrophotometric detection using a high-sensitivity detection cell and solid-phase extraction / $c K. Petrů, J. Siroká, L. Bydžovská, L. Krčmová, M. Polášek,
- 520 9_
- $a A sensitive capillary electrophoretic method featuring spectrophotometric detection using a commercial Z-cell was devised for the assay of 8-hydroxy-2'-deoxyguanosine (8OHdG) in human urine. Solid-phase extraction (SPE) based on hydrophilic-lipophilic-balanced RP sorbent was utilized for urine sample pretreatment and analyte preconcentration. The separation was carried out in conventional fused-silica capillaries employing a Z-cell with hydrodynamic sample injection (at 50 mbar for 12 s). The BGE (pH* 9.2, adjusted with 1 M NaOH) contained 0.15 M boric acid and 10% v/v ACN. The detection wavelength was 282 nm. The calibration curve for 8OHdG (measured in spiked urine) was linear in the range 10-1000 ng/mL; R(2) = 0.9993. The LOD was 3 ng/mL (11 nmol/L) of 8OHdG. Determination of the 8OHdG urinary levels was possible even in healthy individuals.
- 650 _2
- $a deoxyguanosin $x analogy a deriváty $x chemie $x moč $7 D003849
- 650 _2
- $a elektroforéza kapilární $x metody $7 D019075
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a limita detekce $7 D057230
- 650 _2
- $a lineární modely $7 D016014
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 650 _2
- $a extrakce na pevné fázi $x metody $7 D052616
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Siroká, Jitka
- 700 1_
- $a Bydžovská, Lucie
- 700 1_
- $a Krčmová Kujovská, Lenka, $d 1981- $7 xx0209338
- 700 1_
- $a Polášek, Miroslav
- 773 0_
- $w MED00001508 $t Electrophoresis $x 1522-2683 $g Roč. 35, č. 17 (2014), s. 2546-9
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24789757 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150709 $b ABA008
- 991 __
- $a 20161227111545 $b ABA008
- 999 __
- $a ok $b bmc $g 1083781 $s 906437
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 35 $c 17 $d 2546-9 $i 1522-2683 $m Electrophoresis $n Electrophoresis $x MED00001508
- LZP __
- $a Pubmed-20150709
