-
Something wrong with this record ?
In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein
K. Kolostova, Y. Zhang, RM. Hoffman, V. Bobek,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Humans MeSH
- Luminescent Proteins biosynthesis MeSH
- Disease Models, Animal * MeSH
- Mice, Nude MeSH
- Mice MeSH
- Tumor Cells, Cultured MeSH
- Neoplastic Cells, Circulating pathology MeSH
- Lung Neoplasms pathology MeSH
- Porosity MeSH
- Surface Properties MeSH
- Cell Separation MeSH
- Neoplasm Transplantation pathology MeSH
- Particle Size MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In the present study, we demonstrate an animal model and recently introduced size-based exclusion method for circulating tumor cells (CTCs) isolation. The methodology enables subsequent in vitro CTC-culture and characterization. Human lung cancer cell line H460, expressing red fluorescent protein (H460-RFP), was orthotopically implanted in nude mice. CTCs were isolated by a size-based filtration method and successfully cultured in vitro on the separating membrane (MetaCell®), analyzed by means of time-lapse imaging. The cultured CTCs were heterogeneous in size and morphology even though they originated from a single tumor. The outer CTC-membranes were blebbing in general. Abnormal mitosis resulting in three daughter cells was frequently observed. The expression of RFP ensured that the CTCs originated from lung tumor. These readily isolatable, identifiable and cultivable CTCs can be used to characterize individual patient cancers and for screening of more effective treatment.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15031899
- 003
- CZ-PrNML
- 005
- 20151013111754.0
- 007
- ta
- 008
- 151005s2014 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s10895-014-1439-3 $2 doi
- 035 __
- $a (PubMed)25141982
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Kolostova, Katarina $u Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 100 34, Prague, Czech Republic.
- 245 10
- $a In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein / $c K. Kolostova, Y. Zhang, RM. Hoffman, V. Bobek,
- 520 9_
- $a In the present study, we demonstrate an animal model and recently introduced size-based exclusion method for circulating tumor cells (CTCs) isolation. The methodology enables subsequent in vitro CTC-culture and characterization. Human lung cancer cell line H460, expressing red fluorescent protein (H460-RFP), was orthotopically implanted in nude mice. CTCs were isolated by a size-based filtration method and successfully cultured in vitro on the separating membrane (MetaCell®), analyzed by means of time-lapse imaging. The cultured CTCs were heterogeneous in size and morphology even though they originated from a single tumor. The outer CTC-membranes were blebbing in general. Abnormal mitosis resulting in three daughter cells was frequently observed. The expression of RFP ensured that the CTCs originated from lung tumor. These readily isolatable, identifiable and cultivable CTCs can be used to characterize individual patient cancers and for screening of more effective treatment.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a separace buněk $7 D002469
- 650 12
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a luminescentní proteiny $x biosyntéza $7 D008164
- 650 _2
- $a nádory plic $x patologie $7 D008175
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši nahé $7 D008819
- 650 _2
- $a transplantace nádorů $x patologie $7 D009368
- 650 _2
- $a nádorové cirkulující buňky $x patologie $7 D009360
- 650 _2
- $a velikost částic $7 D010316
- 650 _2
- $a poréznost $7 D016062
- 650 _2
- $a povrchové vlastnosti $7 D013499
- 650 _2
- $a nádorové buňky kultivované $7 D014407
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zhang, Yong
- 700 1_
- $a Hoffman, Robert M
- 700 1_
- $a Bobek, Vladimir
- 773 0_
- $w MED00007718 $t Journal of fluorescence $x 1573-4994 $g Roč. 24, č. 5 (2014), s. 1531-6
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25141982 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20151005 $b ABA008
- 991 __
- $a 20151013111943 $b ABA008
- 999 __
- $a ok $b bmc $g 1092775 $s 915025
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 24 $c 5 $d 1531-6 $e 20140822 $i 1573-4994 $m Journal of fluorescence $n J Fluoresc $x MED00007718
- LZP __
- $a Pubmed-20151005
