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Effect of Ca2+ efflux pathway distribution and exogenous Ca2+ buffers on intracellular Ca2+ dynamics in the rat ventricular myocyte: a simulation study
M. Pásek, J. Simurda, CH. Orchard,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT14301
MZ0
CEP - Centrální evidence projektů
NLK
Free Medical Journals
od 2013
PubMed Central
od 2013
Europe PubMed Central
od 2013
ProQuest Central
od 2013
Open Access Digital Library
od 2001-01-01
Open Access Digital Library
od 2012-12-04
Open Access Digital Library
od 2013-01-01
CINAHL Plus with Full Text (EBSCOhost)
od 2013-01-01
Medline Complete (EBSCOhost)
od 2013-01-01
Health & Medicine (ProQuest)
od 2013
Wiley-Blackwell Open Access Titles
od 2001
ROAD: Directory of Open Access Scholarly Resources
od 2013
PubMed
24971358
DOI
10.1155/2014/920208
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- časové faktory MeSH
- EGTA analogy a deriváty farmakologie MeSH
- gating iontového kanálu účinky léků MeSH
- intracelulární prostor účinky léků metabolismus MeSH
- kardiomyocyty účinky léků metabolismus MeSH
- kompartmentace buňky MeSH
- krysa rodu rattus MeSH
- počítačová simulace MeSH
- pufry MeSH
- pumpa pro výměnu sodíku a vápníku metabolismus MeSH
- sarkolema účinky léků metabolismus MeSH
- srdeční komory cytologie MeSH
- vápník metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We have used a previously published computer model of the rat cardiac ventricular myocyte to investigate the effect of changing the distribution of Ca(2+) efflux pathways (SERCA, Na(+)/Ca(2+) exchange, and sarcolemmal Ca(2+) ATPase) between the dyad and bulk cytoplasm and the effect of adding exogenous Ca(2+) buffers (BAPTA or EGTA), which are used experimentally to differentially buffer Ca(2+) in the dyad and bulk cytoplasm, on cellular Ca(2+) cycling. Increasing the dyadic fraction of a particular Ca(2+) efflux pathway increases the amount of Ca(2+) removed by that pathway, with corresponding changes in Ca(2+) efflux from the bulk cytoplasm. The magnitude of these effects varies with the proportion of the total Ca(2+) removed from the cytoplasm by that pathway. Differences in the response to EGTA and BAPTA, including changes in Ca(2+)-dependent inactivation of the L-type Ca(2+) current, resulted from the buffers acting as slow and fast "shuttles," respectively, removing Ca(2+) from the dyadic space. The data suggest that complex changes in dyadic Ca(2+) and cellular Ca(2+) cycling occur as a result of changes in the location of Ca(2+) removal pathways or the presence of exogenous Ca(2+) buffers, although changing the distribution of Ca(2+) efflux pathways has relatively small effects on the systolic Ca(2+) transient.
Department of Physiology Faculty of Medicine Masaryk University Kamenice 5 62500 Brno Czech Republic
School of Physiology and Pharmacology University of Bristol Bristol BS8 1TD UK
Citace poskytuje Crossref.org
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