-
Je něco špatně v tomto záznamu ?
The CRZ1/SP1-like gene links survival under limited aeration, cell integrity and biofilm formation in the pathogenic yeast Cryptococcus neoformans
Z. Moranova, E. Virtudazo, K. Hricova, M. Ohkusu, S. Kawamoto, V. Husickova, V. Raclavsky
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 1998
Medline Complete (EBSCOhost) od 2007-06-01
ROAD: Directory of Open Access Scholarly Resources od 2001
Odkazy
PubMed
23640031
DOI
10.5507/bp.2013.024
domovská stránka časopisu
Knihovny.cz E-zdroje
- MeSH
- anaerobióza MeSH
- antifungální látky farmakologie MeSH
- biofilmy růst a vývoj MeSH
- Cryptococcus neoformans účinky léků genetika růst a vývoj MeSH
- delece genu MeSH
- flukonazol farmakologie MeSH
- kontrolní body buněčného cyklu genetika MeSH
- mikrobiální viabilita MeSH
- proteiny Caenorhabditis elegans genetika MeSH
- transkripční faktory genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
AIMS: Limited aeration has been demonstrated to cause slowdown in proliferation and delayed budding, resulting eventually in a unique unbudded G2-arrest in the obligate aerobic pathogenic yeast Cryptococcus neoformans. Also, the ability to adapt to decreased oxygen levels during pathogenesis has been identified as a virulence factor in C. neoformans. The aim of this study was to identify and characterize genes that are necessary for the proliferation slowdown and G2-arrest caused by limited aeration. METHODS: Random mutants were prepared and screened for lack of typical slowdown of proliferation under limited aeration. The CNAG_00156.2 gene coding for a zinc-finger transcription factor was identified in mutants showing most distinctive phenotype. Targeted deletion strain and reconstituted strain were prepared to characterize and confirm the gene functions. This gene was also identified in a parallel studies as homologous both to calcineurin responsive (Crz1) and PKC1-dependent (SP1-like) transcription factors. RESULTS: We have confirmed the role of the cryptococcal homologue of CRZ1/SP1-like transcription factor in cell integrity, and newly demonstrated its role in slowdown of proliferation and survival under reduced aeration, in biofilm formation and in susceptibility to fluconazole. CONCLUSIONS: Our data demonstrate a tight molecular link between slowdown of proliferation during hypoxic adaptation and maintenance of cell integrity in C. neoformans and present a new role for the CRZ1 family of transcription factors in fungi. The exact positioning of this protein in cryptococcal signalling cascades remains to be clarified.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15033234
- 003
- CZ-PrNML
- 005
- 20151030145017.0
- 007
- ta
- 008
- 151016s2014 xr ad f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.5507/bp.2013.024 $2 doi
- 035 __
- $a (PubMed)23640031
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Moráňová, Zuzana $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic; Division of Molecular Biology, Medical Mycology Research Center, Chiba University, Chiba, Japan $7 _AN042513
- 245 14
- $a The CRZ1/SP1-like gene links survival under limited aeration, cell integrity and biofilm formation in the pathogenic yeast Cryptococcus neoformans / $c Z. Moranova, E. Virtudazo, K. Hricova, M. Ohkusu, S. Kawamoto, V. Husickova, V. Raclavsky
- 520 9_
- $a AIMS: Limited aeration has been demonstrated to cause slowdown in proliferation and delayed budding, resulting eventually in a unique unbudded G2-arrest in the obligate aerobic pathogenic yeast Cryptococcus neoformans. Also, the ability to adapt to decreased oxygen levels during pathogenesis has been identified as a virulence factor in C. neoformans. The aim of this study was to identify and characterize genes that are necessary for the proliferation slowdown and G2-arrest caused by limited aeration. METHODS: Random mutants were prepared and screened for lack of typical slowdown of proliferation under limited aeration. The CNAG_00156.2 gene coding for a zinc-finger transcription factor was identified in mutants showing most distinctive phenotype. Targeted deletion strain and reconstituted strain were prepared to characterize and confirm the gene functions. This gene was also identified in a parallel studies as homologous both to calcineurin responsive (Crz1) and PKC1-dependent (SP1-like) transcription factors. RESULTS: We have confirmed the role of the cryptococcal homologue of CRZ1/SP1-like transcription factor in cell integrity, and newly demonstrated its role in slowdown of proliferation and survival under reduced aeration, in biofilm formation and in susceptibility to fluconazole. CONCLUSIONS: Our data demonstrate a tight molecular link between slowdown of proliferation during hypoxic adaptation and maintenance of cell integrity in C. neoformans and present a new role for the CRZ1 family of transcription factors in fungi. The exact positioning of this protein in cryptococcal signalling cascades remains to be clarified.
- 650 _2
- $a anaerobióza $7 D000693
- 650 _2
- $a antifungální látky $x farmakologie $7 D000935
- 650 _2
- $a biofilmy $x růst a vývoj $7 D018441
- 650 _2
- $a proteiny Caenorhabditis elegans $x genetika $7 D029742
- 650 _2
- $a kontrolní body buněčného cyklu $x genetika $7 D059447
- 650 _2
- $a Cryptococcus neoformans $x účinky léků $x genetika $x růst a vývoj $7 D003455
- 650 _2
- $a flukonazol $x farmakologie $7 D015725
- 650 _2
- $a delece genu $7 D017353
- 650 _2
- $a mikrobiální viabilita $7 D050296
- 650 _2
- $a transkripční faktory $x genetika $7 D014157
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Virtudazo, Eric $u Division of Molecular Biology, Medical Mycology Research Center, Chiba University, Chiba, Japan
- 700 1_
- $a Hricová, Kristýna $7 xx0267923 $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
- 700 1_
- $a Ohkusu, Misako $u Division of Molecular Biology, Medical Mycology Research Center, Chiba University, Chiba, Japan
- 700 1_
- $a Kawamoto, Susumu $u Division of Molecular Biology, Medical Mycology Research Center, Chiba University, Chiba, Japan
- 700 1_
- $a Husičková, Vendula $7 _AN042512 $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
- 700 1_
- $a Raclavský, Vladislav $7 xx0046827 $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
- 773 0_
- $w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic $x 1213-8118 $g Roč. 158, č. 2 (2014), s. 212-220
- 856 41
- $u http://biomed.papers.upol.cz/ $y domovská stránka časopisu
- 910 __
- $a ABA008 $b A 1502 $c 958 $y 4 $z 0
- 990 __
- $a 20151016 $b ABA008
- 991 __
- $a 20151027141742 $b ABA008
- 999 __
- $a ok $b bmc $g 1095632 $s 916365
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 158 $c 2 $d 212-220 $e 20130422 $i 1213-8118 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606
- LZP __
- $b NLK118 $a Pubmed-20151016