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High-resolution melting analysis for identifying sequence variations in nuclear genes for assembly factors and structural subunits of cytochrome c oxidase
A. Vondráčková, K. Veselá, J. Zeman, M. Tesařová,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Genetic Variation * MeSH
- Genotype MeSH
- Humans MeSH
- Nuclear Magnetic Resonance, Biomolecular methods MeSH
- Protein Subunits chemistry genetics metabolism MeSH
- Polymerase Chain Reaction methods MeSH
- Electron Transport Complex IV chemistry metabolism MeSH
- Nucleic Acid Amplification Techniques * methods MeSH
- Transition Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
High-resolution melting (HRM) analysis is a simple, sensitive, and cost-effective screening method. HRM enables the detection of homozygous or heterozygous point sequence variants and small deletions within specific PCR products by observing temperature and shape changes in melting curve profiles using fluorescent dyes. Herein, an updated protocol for routine variant screening of nuclear genes encoding assembly factors and structural subunits of cytochrome c oxidase (COX) is described. Nonetheless, the general recommendations given for HRM analysis can be applicable for examining any genetic region of interest.
References provided by Crossref.org
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