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In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp
B. Brežná, J. Šmíd, J. Costa, J. Radvanszky, I. Mafra, T. Kuchta,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Odkazy
PubMed
25483259
DOI
10.1016/j.mcp.2014.11.006
Knihovny.cz E-zdroje
- MeSH
- DNA sondy metabolismus MeSH
- listy rostlin MeSH
- počítačová simulace MeSH
- polymerázová řetězová reakce metody MeSH
- rostlinné proteiny genetika MeSH
- slivoň klasifikace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silico evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Röder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Röder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond.
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