-
Je něco špatně v tomto záznamu ?
Biotransformation of anthelmintics and the activity of drug-metabolizing enzymes in the tapeworm Moniezia expansa
L. Prchal, H. Bártíková, A. Bečanová, R. Jirásko, I. Vokřál, L. Stuchlíková, L. Skálová, V. Kubíček, J. Lamka, F. Trejtnar, B. Szotáková,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 2001-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2001-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 2001-01-01 do Před 1 rokem
ROAD: Directory of Open Access Scholarly Resources
od 1908
- MeSH
- albendazol farmakokinetika farmakologie MeSH
- alkoholoxidoreduktasy metabolismus MeSH
- anthelmintika farmakokinetika farmakologie MeSH
- biotransformace MeSH
- Cestoda účinky léků enzymologie ultrastruktura MeSH
- glukuronosyltransferasa metabolismus MeSH
- glutathiontransferasa metabolismus MeSH
- izoenzymy metabolismus MeSH
- katalasa metabolismus MeSH
- mebendazol analogy a deriváty farmakokinetika farmakologie MeSH
- monieziáza parazitologie MeSH
- multienzymové komplexy metabolismus MeSH
- NADH, NADPH oxidoreduktasy metabolismus MeSH
- nemoci ovcí parazitologie MeSH
- ovce MeSH
- oxidace-redukce MeSH
- oxygenasy se smíšenou funkcí metabolismus MeSH
- peroxidasa metabolismus MeSH
- superoxiddismutasa metabolismus MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- tenké střevo parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The sheep tapeworm Moniezia expansa is very common parasite, which affects ruminants such as sheep, goats as well as other species. The benzimidazole anthelmintics albendazole (ABZ), flubendazole (FLU) and mebendazole (MBZ) are often used to treat the infection. The drug-metabolizing enzymes of helminths may alter the potency of anthelmintic treatment. The aim of our study was to assess the activity of the main drug-metabolizing enzymes and evaluate the metabolism of selected anthelmintics (ABZ, MBZ and FLU) in M. expansa. Activities of biotransformation enzymes were determined in subcellular fractions. Metabolites of the anthelmintics were detected and identified using high performance liquid chromatography/ultra-violet/VIS/fluorescence or ultra-high performance liquid chromatography/mass spectrometry. Reduction of MBZ, FLU and oxidation of ABZ were proved as well as activities of various metabolizing enzymes. Despite the fact that the conjugation enzymes glutathione S-transferase, UDP-glucuronosyl transferase and UDP-glucosyl transferase were active in vitro, no conjugated metabolites of anthelmintics were identified either ex vivo or in vitro. The obtained results indicate that sheep tapeworm is able to deactivate the administered anthelmintics, and thus protects itself against their action.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16000552
- 003
- CZ-PrNML
- 005
- 20160127095228.0
- 007
- ta
- 008
- 160108s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1017/S0031182014001711 $2 doi
- 035 __
- $a (PubMed)25373326
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Prchal, Lukáš $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 245 10
- $a Biotransformation of anthelmintics and the activity of drug-metabolizing enzymes in the tapeworm Moniezia expansa / $c L. Prchal, H. Bártíková, A. Bečanová, R. Jirásko, I. Vokřál, L. Stuchlíková, L. Skálová, V. Kubíček, J. Lamka, F. Trejtnar, B. Szotáková,
- 520 9_
- $a The sheep tapeworm Moniezia expansa is very common parasite, which affects ruminants such as sheep, goats as well as other species. The benzimidazole anthelmintics albendazole (ABZ), flubendazole (FLU) and mebendazole (MBZ) are often used to treat the infection. The drug-metabolizing enzymes of helminths may alter the potency of anthelmintic treatment. The aim of our study was to assess the activity of the main drug-metabolizing enzymes and evaluate the metabolism of selected anthelmintics (ABZ, MBZ and FLU) in M. expansa. Activities of biotransformation enzymes were determined in subcellular fractions. Metabolites of the anthelmintics were detected and identified using high performance liquid chromatography/ultra-violet/VIS/fluorescence or ultra-high performance liquid chromatography/mass spectrometry. Reduction of MBZ, FLU and oxidation of ABZ were proved as well as activities of various metabolizing enzymes. Despite the fact that the conjugation enzymes glutathione S-transferase, UDP-glucuronosyl transferase and UDP-glucosyl transferase were active in vitro, no conjugated metabolites of anthelmintics were identified either ex vivo or in vitro. The obtained results indicate that sheep tapeworm is able to deactivate the administered anthelmintics, and thus protects itself against their action.
- 650 _2
- $a albendazol $x farmakokinetika $x farmakologie $7 D015766
- 650 _2
- $a alkoholoxidoreduktasy $x metabolismus $7 D000429
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a anthelmintika $x farmakokinetika $x farmakologie $7 D000871
- 650 _2
- $a biotransformace $7 D001711
- 650 _2
- $a katalasa $x metabolismus $7 D002374
- 650 _2
- $a Cestoda $x účinky léků $x enzymologie $x ultrastruktura $7 D002589
- 650 _2
- $a systém (enzymů) cytochromů P-450 $x metabolismus $7 D003577
- 650 _2
- $a glukuronosyltransferasa $x metabolismus $7 D014453
- 650 _2
- $a glutathiontransferasa $x metabolismus $7 D005982
- 650 _2
- $a tenké střevo $x parazitologie $7 D007421
- 650 _2
- $a izoenzymy $x metabolismus $7 D007527
- 650 _2
- $a mebendazol $x analogy a deriváty $x farmakokinetika $x farmakologie $7 D008463
- 650 _2
- $a oxygenasy se smíšenou funkcí $x metabolismus $7 D006899
- 650 _2
- $a monieziáza $x parazitologie $7 D008989
- 650 _2
- $a multienzymové komplexy $x metabolismus $7 D009097
- 650 _2
- $a NADH, NADPH oxidoreduktasy $x metabolismus $7 D009247
- 650 _2
- $a oxidace-redukce $7 D010084
- 650 _2
- $a peroxidasa $x metabolismus $7 D009195
- 650 _2
- $a ovce $7 D012756
- 650 _2
- $a nemoci ovcí $x parazitologie $7 D012757
- 650 _2
- $a superoxiddismutasa $x metabolismus $7 D013482
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Bártíková, Hana $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Bečanová, Aneta $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Jirásko, Robert $u Faculty of Chemical Technology,University of Pardubice,Studentská 573,CZ-53210 Pardubice,Czech Republic.
- 700 1_
- $a Vokřál, Ivan $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Stuchlíková, Lucie $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Skálová, Lenka $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Kubíček, Vladimír $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Lamka, Jiří $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Trejtnar, František $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 700 1_
- $a Szotáková, Barbora $u Faculty of Pharmacy,Charles University,Heyrovského 1203,CZ-50005 Hradec Králové,Czech Republic.
- 773 0_
- $w MED00010377 $t Parasitology $x 1469-8161 $g Roč. 142, č. 5 (2015), s. 648-59
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25373326 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160108 $b ABA008
- 991 __
- $a 20160127095353 $b ABA008
- 999 __
- $a ok $b bmc $g 1102833 $s 924758
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 142 $c 5 $d 648-59 $e 20141106 $i 1469-8161 $m Parasitology $n Parasitology $x MED00010377
- LZP __
- $a Pubmed-20160108
