Cytokinins (CKs) regulate plant development and growth via a two-component signaling pathway. By forward genetic screening, we isolated an Arabidopsis mutant named grow fast on cytokinins 1 (gfc1), whose seedlings grew larger aerial parts on MS medium with CK. gfc1 is allelic to a previously reported cutin mutant defective in cuticular ridges (dcr). GFC1/DCR encodes a soluble BAHD acyltransferase (a name based on the first four enzymes characterized in this family: Benzylalcohol O-acetyltransferase, Anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase and Deacetylvindoline 4-O-acetyltransferase) with diacylglycerol acyltransferase (DGAT) activity in vitro and is necessary for normal cuticle formation on epidermis in vivo. Here we show that gfc1 was a CK-insensitive mutant, as revealed by its low regeneration frequency in vitro and resistance to CK in adventitious root formation and dark-grown hypocotyl inhibition assays. In addition, gfc1 had de-etiolated phenotypes in darkness and was therefore defective in skotomorphogenesis. The background expression levels of most type-A Arabidopsis Response Regulator (ARR) genes were higher in the gfc1 mutant. The gfc1-associated phenotypes were also observed in the cutin-deficient glycerol-3-phosphate acyltransferase 4/8 (gpat4/8) double mutant [defective in glycerol-3-phosphate (G3P) acyltransferase enzymes GPAT4 and GPAT8, which redundantly catalyze the acylation of G3P by hydroxyl fatty acid (OH-FA)], but not in the cutin-deficient mutant cytochrome p450, family 86, subfamily A, polypeptide 2/aberrant induction of type three 1 (cyp86A2/att1), which affects the biosynthesis of some OH-FAs. Our results indicate that some acyltransferases associated with cutin formation are involved in CK responses and skotomorphogenesis in Arabidopsis.

Laboratory of Growth Regulators Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany ASCR and Faculty of Science Palacký University Šlechtitelů 11 Olomouc Czech Republic

Laboratory of Molecular and Cell Biology Hebei Normal University Shijiazhuang China

MOE Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou University Lanzhou China

000      

00000naa a2200000 a 4500

001      

bmc16010417

003      

CZ-PrNML

005      

20160408112336.0

007      

ta

008      

160408s2015 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1371/journal.pone.0121943 $2 doi

024    7_

$a 10.1371/journal.pone.0121943 $2 doi

035    __

$a (PubMed)25803274

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Wu, Lei $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

245    10

$a Functional roles of three cutin biosynthetic acyltransferases in cytokinin responses and skotomorphogenesis / $c L. Wu, ZY. Zhou, CG. Zhang, J. Chai, Q. Zhou, L. Wang, E. Hirnerová, M. Mrvková, O. Novák, GQ. Guo,

520    9_

$a Cytokinins (CKs) regulate plant development and growth via a two-component signaling pathway. By forward genetic screening, we isolated an Arabidopsis mutant named grow fast on cytokinins 1 (gfc1), whose seedlings grew larger aerial parts on MS medium with CK. gfc1 is allelic to a previously reported cutin mutant defective in cuticular ridges (dcr). GFC1/DCR encodes a soluble BAHD acyltransferase (a name based on the first four enzymes characterized in this family: Benzylalcohol O-acetyltransferase, Anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase and Deacetylvindoline 4-O-acetyltransferase) with diacylglycerol acyltransferase (DGAT) activity in vitro and is necessary for normal cuticle formation on epidermis in vivo. Here we show that gfc1 was a CK-insensitive mutant, as revealed by its low regeneration frequency in vitro and resistance to CK in adventitious root formation and dark-grown hypocotyl inhibition assays. In addition, gfc1 had de-etiolated phenotypes in darkness and was therefore defective in skotomorphogenesis. The background expression levels of most type-A Arabidopsis Response Regulator (ARR) genes were higher in the gfc1 mutant. The gfc1-associated phenotypes were also observed in the cutin-deficient glycerol-3-phosphate acyltransferase 4/8 (gpat4/8) double mutant [defective in glycerol-3-phosphate (G3P) acyltransferase enzymes GPAT4 and GPAT8, which redundantly catalyze the acylation of G3P by hydroxyl fatty acid (OH-FA)], but not in the cutin-deficient mutant cytochrome p450, family 86, subfamily A, polypeptide 2/aberrant induction of type three 1 (cyp86A2/att1), which affects the biosynthesis of some OH-FAs. Our results indicate that some acyltransferases associated with cutin formation are involved in CK responses and skotomorphogenesis in Arabidopsis.

650    _2

$a acyltransferasy $x genetika $x metabolismus $7 D000217

650    _2

$a Arabidopsis $x genetika $x růst a vývoj $x metabolismus $x účinky záření $7 D017360

650    _2

$a proteiny huseníčku $x genetika $x metabolismus $7 D029681

650    _2

$a cytokininy $x metabolismus $x farmakologie $7 D003583

650    _2

$a tma $7 D003624

650    _2

$a regulace genové exprese u rostlin $x účinky léků $x účinky záření $7 D018506

650    _2

$a membránové lipidy $x biosyntéza $7 D008563

650    _2

$a meristém $x účinky léků $x genetika $x růst a vývoj $x účinky záření $7 D018519

650    12

$a morfogeneze $x účinky léků $x účinky záření $7 D009024

650    _2

$a mutace $7 D009154

650    _2

$a fenotyp $7 D010641

650    _2

$a semenáček $x účinky léků $x genetika $x růst a vývoj $x účinky záření $7 D036226

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Zhou, Zhao-Yang $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

700    1_

$a Zhang, Chun-Guang $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China; Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang, China.

700    1_

$a Chai, Juan $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

700    1_

$a Zhou, Qin $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

700    1_

$a Wang, Li $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

700    1_

$a Hirnerová, Eva $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR & Faculty of Science, Palacký University, Šlechtitelů 11, Olomouc, Czech Republic.

700    1_

$a Mrvková, Michaela $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR & Faculty of Science, Palacký University, Šlechtitelů 11, Olomouc, Czech Republic.

700    1_

$a Novák, Ondřej $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR & Faculty of Science, Palacký University, Šlechtitelů 11, Olomouc, Czech Republic.

700    1_

$a Guo, Guang-Qin $u MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

773    0_

$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 10, č. 3 (2015), s. e0121943

856    41

$u https://pubmed.ncbi.nlm.nih.gov/25803274 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20160408 $b ABA008

991    __

$a 20160408112414 $b ABA008

999    __

$a ok $b bmc $g 1113846 $s 934785

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2015 $b 10 $c 3 $d e0121943 $e 20150324 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950

LZP    __

$a Pubmed-20160408