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Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
M. Sittová, M. Röderová, M. Dendis, K. Hricová, V. Pudová, R. Horváth, F. Růžička, Š. Dosoudilová, M. Kolář,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2000-06-01 to 2018-12-31
Health & Medicine (ProQuest)
from 2000-06-01 to 2018-12-31
Public Health Database (ProQuest)
from 2000-06-01 to 2018-12-31
PubMed
25588196
DOI
10.1089/mdr.2014.0210
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- beta-Lactamases genetics metabolism MeSH
- beta-Lactam Resistance genetics MeSH
- Enterobacteriaceae drug effects genetics isolation & purification MeSH
- Enterobacteriaceae Infections diagnosis drug therapy microbiology MeSH
- Gene Expression MeSH
- Respiratory Tract Infections diagnosis drug therapy microbiology MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Plasmids chemistry metabolism MeSH
- Retrospective Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The infections caused by extended-spectrum β-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
References provided by Crossref.org
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