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Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
M. Sittová, M. Röderová, M. Dendis, K. Hricová, V. Pudová, R. Horváth, F. Růžička, Š. Dosoudilová, M. Kolář,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2000-06-01 do 2018-12-31
Health & Medicine (ProQuest)
od 2000-06-01 do 2018-12-31
Public Health Database (ProQuest)
od 2000-06-01 do 2018-12-31
PubMed
25588196
DOI
10.1089/mdr.2014.0210
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- beta-laktamasy genetika metabolismus MeSH
- beta-laktamová rezistence genetika MeSH
- Enterobacteriaceae účinky léků genetika izolace a purifikace MeSH
- enterobakteriální infekce diagnóza farmakoterapie mikrobiologie MeSH
- exprese genu MeSH
- infekce dýchací soustavy diagnóza farmakoterapie mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- plazmidy chemie metabolismus MeSH
- retrospektivní studie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The infections caused by extended-spectrum β-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
Citace poskytuje Crossref.org
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