Bacterial and fungal biodiversity throughout different biostimulation and bioaugmentation treatments applied to an industrial creosote-polluted soil were analyzed by means of polyphasic approach in order to gain insight into the microbial community structure and dynamics. Pyrosequencing data obtained from initial creosote polluted soil (after a biopiling step) revealed that Alpha and Gammaproteobacteria were the most abundant bacterial groups, whereas Fusarium and Scedosporium were the main fungal genera in the contaminated soil. At the end of 60-days laboratory scale bioremediation assays, pyrosequencing and DGGE data showed that (i) major bacterial community shifts were caused by the type of mobilizing agent added to the soil and, to a lesser extent, by the addition of lignocellulosic substrate; and (ii) the presence of the non-ionic surfactant (Brij 30) hampered the proliferation of Actinobacteria (Mycobacteriaceae) and Bacteroidetes (Chitinophagaceae) and, in the absence of lignocellulosic substrate, also impeded polycyclic aromatic hydrocarbons (PAHs) degradation. The results show the importance of implementing bioremediation experiments combined with microbiome assessment to gain insight on the effect of crucial parameters (e.g. use of additives) over the potential functions of complex microbial communities harbored in polluted soils, essential for bioremediation success.

Department for Innovation in Biological Agro Food and Forest Systems [DIBAF] University of Tuscia Via S Camillo de Lellis snc 01100 Viterbo Italy

Department of Microbiology University of Barcelona Diagonal 645 E 08028 Barcelona Spain

GIRO Joint Research Unit IRTA UPC Institute of Research and Technology Food and Agriculture [IRTA] Torre Marimon E 08140 Caldes de Montbui Spain

Institute of Microbiology Academy of Sciences of the Czech Republic Vídenská 1083 142 20 Prague 4 Czech Republic

Citace poskytuje Crossref.org