A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
23156141
DOI
10.1603/ec12010
Knihovny.cz E-zdroje
- MeSH
- metody pro podporu rozhodování MeSH
- nukleové kyseliny analýza MeSH
- Paenibacillus izolace a purifikace fyziologie MeSH
- počítačová simulace MeSH
- polymerázová řetězová reakce ekonomika metody MeSH
- senzitivita a specificita MeSH
- spory bakteriální izolace a purifikace fyziologie MeSH
- včely mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nukleové kyseliny MeSH
American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less).
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