A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris
Language English Country England, Great Britain Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
23156141
DOI
10.1603/ec12010
Knihovny.cz E-resources
- MeSH
- Decision Support Techniques MeSH
- Nucleic Acids analysis MeSH
- Paenibacillus isolation & purification physiology MeSH
- Computer Simulation MeSH
- Polymerase Chain Reaction economics methods MeSH
- Sensitivity and Specificity MeSH
- Spores, Bacterial isolation & purification physiology MeSH
- Bees microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nucleic Acids MeSH
American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less).
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