This study presents synthesis of paramagnetic particles formed by nanomaghemite (γ-Fe2O3) core, whose surface was modified with LaCl3 and entire composite is able to specifically bind the bacteriophage λ. In this study we described the structure of bacteriophage λ, its biochemical properties and role in the therapy, biotechnology and nanomedicine. In the next part we summarized accessible information about paramagnetic particles, their attributes and specificity of binding the bacteriophage λ. Paramagnetic particles show the ability to immobilize bacteriophage λ for subsequent analysis using ion-exchange chromatography with Vis detection. The presence of bacteriophage λ on the surface of paramagnetic beads was demonstrated by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE). It can be stated that paramagnetic beads show significant binding ability towards bacteriophage λ. This phenomenon can be employed for magnetic separation of bacteriophages prior their possible modifications and subsequent application as a cytostatic nanotransporters or antimicrobial agents usable in hard-to-heal bacterial infections.

Central European Institute of Technology Brno University of Technology Czech Republic European Union

Department of Chemistry and Biochemistry Laboratory of Metallomics and Nanotechnologies Mendel University in Brno Czech Republic European Union