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Correlative Fluorescence and Scanning Electron Microscopy of Labelled Core Fucosylated Glycans Using Cryosections Mounted on Carbon-Patterned Glass Slides
M. Vancová, J. Nebesářová,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
Free Medical Journals
od 2006
Public Library of Science (PLoS)
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PubMed Central
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ProQuest Central
od 2006-12-01
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od 2006-01-01
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od 2006-10-01
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od 2006-01-01
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od 2008-01-01
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od 2006-12-01
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od 2006-12-01
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od 2006-12-01
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od 2006
- MeSH
- barvení a značení metody MeSH
- elektronová kryomikroskopie přístrojové vybavení metody MeSH
- fluorescenční mikroskopie přístrojové vybavení metody MeSH
- klíště * metabolismus ultrastruktura MeSH
- mikroskopie elektronová rastrovací přístrojové vybavení metody MeSH
- proteiny členovců metabolismus MeSH
- proteoglykany metabolismus MeSH
- sklo MeSH
- slinné proteiny a peptidy metabolismus MeSH
- slinné žlázy * metabolismus ultrastruktura MeSH
- uhlík MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of the study is co-localization of N-glycans with fucose attached to N-acetylglucosamine in α1,3 linkage, that belong to immunogenic carbohydrate epitopes in humans, and N-glycans with α1,6-core fucose typical for mammalian type of N-linked glycosylation. Both glycan epitopes were labelled in cryosections of salivary glands isolated from the tick Ixodes ricinus. Salivary glands secrete during feeding many bioactive molecules and influence both successful feeding and transmission of tick-borne pathogens. For accurate and reliable localization of labelled glycans in both fluorescence and scanning electron microscopes, we used carbon imprints of finder or indexed EM grids on glass slides. We discuss if the topographical images can provide information about labelled structures, the working setting of the field-emission scanning electron microscope and the influence of the detector selection (a below-the-lens Autrata improved YAG detector of back-scattered electrons; in-lens and conventional Everhart-Thornley detectors of secondary electrons) on the imaging of gold nanoparticles, quantum dots and osmium-stained membranes.
Citace poskytuje Crossref.org
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