Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Correlative Fluorescence and Scanning Electron Microscopy of Labelled Core Fucosylated Glycans Using Cryosections Mounted on Carbon-Patterned Glass Slides

M. Vancová, J. Nebesářová,

. 2015 ; 10 (12) : e0145034. [pub] 20151221

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16020079

The aim of the study is co-localization of N-glycans with fucose attached to N-acetylglucosamine in α1,3 linkage, that belong to immunogenic carbohydrate epitopes in humans, and N-glycans with α1,6-core fucose typical for mammalian type of N-linked glycosylation. Both glycan epitopes were labelled in cryosections of salivary glands isolated from the tick Ixodes ricinus. Salivary glands secrete during feeding many bioactive molecules and influence both successful feeding and transmission of tick-borne pathogens. For accurate and reliable localization of labelled glycans in both fluorescence and scanning electron microscopes, we used carbon imprints of finder or indexed EM grids on glass slides. We discuss if the topographical images can provide information about labelled structures, the working setting of the field-emission scanning electron microscope and the influence of the detector selection (a below-the-lens Autrata improved YAG detector of back-scattered electrons; in-lens and conventional Everhart-Thornley detectors of secondary electrons) on the imaging of gold nanoparticles, quantum dots and osmium-stained membranes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020079
003      
CZ-PrNML
005      
20160722115927.0
007      
ta
008      
160722s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0145034 $2 doi
024    7_
$a 10.1371/journal.pone.0145034 $2 doi
035    __
$a (PubMed)26690057
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vancová, Marie $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic. Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
245    10
$a Correlative Fluorescence and Scanning Electron Microscopy of Labelled Core Fucosylated Glycans Using Cryosections Mounted on Carbon-Patterned Glass Slides / $c M. Vancová, J. Nebesářová,
520    9_
$a The aim of the study is co-localization of N-glycans with fucose attached to N-acetylglucosamine in α1,3 linkage, that belong to immunogenic carbohydrate epitopes in humans, and N-glycans with α1,6-core fucose typical for mammalian type of N-linked glycosylation. Both glycan epitopes were labelled in cryosections of salivary glands isolated from the tick Ixodes ricinus. Salivary glands secrete during feeding many bioactive molecules and influence both successful feeding and transmission of tick-borne pathogens. For accurate and reliable localization of labelled glycans in both fluorescence and scanning electron microscopes, we used carbon imprints of finder or indexed EM grids on glass slides. We discuss if the topographical images can provide information about labelled structures, the working setting of the field-emission scanning electron microscope and the influence of the detector selection (a below-the-lens Autrata improved YAG detector of back-scattered electrons; in-lens and conventional Everhart-Thornley detectors of secondary electrons) on the imaging of gold nanoparticles, quantum dots and osmium-stained membranes.
650    _2
$a zvířata $7 D000818
650    _2
$a proteiny členovců $x metabolismus $7 D060829
650    _2
$a uhlík $7 D002244
650    _2
$a elektronová kryomikroskopie $x přístrojové vybavení $x metody $7 D020285
650    _2
$a sklo $7 D005898
650    12
$a klíště $x metabolismus $x ultrastruktura $7 D018884
650    _2
$a mikroskopie elektronová rastrovací $x přístrojové vybavení $x metody $7 D008855
650    _2
$a fluorescenční mikroskopie $x přístrojové vybavení $x metody $7 D008856
650    _2
$a proteoglykany $x metabolismus $7 D011509
650    12
$a slinné žlázy $x metabolismus $x ultrastruktura $7 D012469
650    _2
$a slinné proteiny a peptidy $x metabolismus $7 D012471
650    _2
$a barvení a značení $x metody $7 D013194
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Nebesářová, Jana $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic. Faculty of Science, Charles University in Prague, Prague, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 10, č. 12 (2015), s. e0145034
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26690057 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160722120142 $b ABA008
999    __
$a ok $b bmc $g 1154749 $s 944607
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 10 $c 12 $d e0145034 $e 20151221 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20160722

Najít záznam

Citační ukazatele

Možnosti archivace