-
Something wrong with this record ?
The speciation and genotyping of Cronobacter isolates from hospitalised patients
A. Alsonosi, S. Hariri, M. Kajsík, M. Oriešková, V. Hanulík, M. Röderová, J. Petrželová, H. Kollárová, H. Drahovská, S. Forsythe, O. Holý,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- Cronobacter genetics isolation & purification MeSH
- Child MeSH
- Adult MeSH
- Feces microbiology MeSH
- Genotype * MeSH
- Inpatients MeSH
- Cohort Studies MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Multilocus Sequence Typing * MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Sputum microbiology MeSH
- Bacterial Typing Techniques MeSH
- Genetic Speciation * MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Infant, Newborn MeSH
- Child, Preschool MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The World Health Organization (WHO) has recognised all Cronobacter species as human pathogens. Among premature neonates and immunocompromised infants, these infections can be life-threatening, with clinical presentations of septicaemia, meningitis and necrotising enterocolitis. The neurological sequelae can be permanent and the mortality rate as high as 40-80%. Despite the highlighted issues of neonatal infections, the majority of Cronobacter infections are in the elderly population suffering from serious underlying disease or malignancy and include wound and urinary tract infections, osteomyelitis, bacteraemia and septicaemia. However, no age profiling studies have speciated or genotyped the Cronobacter isolates. A clinical collection of 51 Cronobacter strains from two hospitals were speciated and genotyped using 7-loci multilocus sequence typing (MLST), rpoB gene sequence analysis, O-antigen typing and pulsed-field gel electrophoresis (PFGE). The isolates were predominated by C. sakazakii sequence type 4 (63%, 32/51) and C. malonaticus sequence type 7 (33%, 17/51). These had been isolated from throat and sputum samples of all age groups, as well as recal and faecal swabs. There was no apparent relatedness between the age of the patient and the Cronobacter species isolated. Despite the high clonality of Cronobacter, PFGE profiles differentiated strains across the sequence types into 15 pulsotypes. There was almost complete agreement between O-antigen typing and rpoB gene sequence analysis and MLST profiling. This study shows the value of applying MLST to bacterial population studies with strains from two patient cohorts, combined with PFGE for further discrimination of strains.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020542
- 003
- CZ-PrNML
- 005
- 20180307074841.0
- 007
- ta
- 008
- 160722s2015 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s10096-015-2440-8 $2 doi
- 024 7_
- $a 10.1007/s10096-015-2440-8 $2 doi
- 035 __
- $a (PubMed)26173692
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Alsonosi, A $u Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK. $7 gn_A_00004861
- 245 14
- $a The speciation and genotyping of Cronobacter isolates from hospitalised patients / $c A. Alsonosi, S. Hariri, M. Kajsík, M. Oriešková, V. Hanulík, M. Röderová, J. Petrželová, H. Kollárová, H. Drahovská, S. Forsythe, O. Holý,
- 520 9_
- $a The World Health Organization (WHO) has recognised all Cronobacter species as human pathogens. Among premature neonates and immunocompromised infants, these infections can be life-threatening, with clinical presentations of septicaemia, meningitis and necrotising enterocolitis. The neurological sequelae can be permanent and the mortality rate as high as 40-80%. Despite the highlighted issues of neonatal infections, the majority of Cronobacter infections are in the elderly population suffering from serious underlying disease or malignancy and include wound and urinary tract infections, osteomyelitis, bacteraemia and septicaemia. However, no age profiling studies have speciated or genotyped the Cronobacter isolates. A clinical collection of 51 Cronobacter strains from two hospitals were speciated and genotyped using 7-loci multilocus sequence typing (MLST), rpoB gene sequence analysis, O-antigen typing and pulsed-field gel electrophoresis (PFGE). The isolates were predominated by C. sakazakii sequence type 4 (63%, 32/51) and C. malonaticus sequence type 7 (33%, 17/51). These had been isolated from throat and sputum samples of all age groups, as well as recal and faecal swabs. There was no apparent relatedness between the age of the patient and the Cronobacter species isolated. Despite the high clonality of Cronobacter, PFGE profiles differentiated strains across the sequence types into 15 pulsotypes. There was almost complete agreement between O-antigen typing and rpoB gene sequence analysis and MLST profiling. This study shows the value of applying MLST to bacterial population studies with strains from two patient cohorts, combined with PFGE for further discrimination of strains.
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a senioři nad 80 let $7 D000369
- 650 _2
- $a techniky typizace bakterií $7 D015373
- 650 _2
- $a dítě $7 D002648
- 650 _2
- $a předškolní dítě $7 D002675
- 650 _2
- $a kohortové studie $7 D015331
- 650 _2
- $a Cronobacter $x genetika $x izolace a purifikace $7 D059126
- 650 _2
- $a pulzní gelová elektroforéza $7 D016521
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a vznik druhů (genetika) $7 D049810
- 650 12
- $a genotyp $7 D005838
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kojenec $7 D007223
- 650 _2
- $a novorozenec $7 D007231
- 650 _2
- $a hospitalizovaní pacienti $7 D007297
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 12
- $a multilokusová sekvenční typizace $7 D058885
- 650 _2
- $a sputum $x mikrobiologie $7 D013183
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hariri, S $u Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK.
- 700 1_
- $a Kajsík, M $u Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
- 700 1_
- $a Oriešková, M $u Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
- 700 1_
- $a Hanulík, V $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic.
- 700 1_
- $a Röderová, Magdalena $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic. $7 xx0106226
- 700 1_
- $a Petrželová, J $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic.
- 700 1_
- $a Kollárová, H $u Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic.
- 700 1_
- $a Drahovská, H $u Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
- 700 1_
- $a Forsythe, S $u Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK. stephen.forsythe@ntu.ac.uk.
- 700 1_
- $a Holý, Ondřej $u Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic. $7 xx0222173
- 773 0_
- $w MED00001612 $t European journal of clinical microbiology & infectious diseases official publication of the European Society of Clinical Microbiology $x 1435-4373 $g Roč. 34, č. 10 (2015), s. 1979-88
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26173692 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20180307074842 $b ABA008
- 999 __
- $a ok $b bmc $g 1155212 $s 945070
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 34 $c 10 $d 1979-88 $e 20150715 $i 1435-4373 $m European journal of clinical microbiology & infectious diseases $n Eur J Clin Microbiol Infect Dis $x MED00001612
- LZP __
- $a Pubmed-20160722
