Following the COVID-19 infection, the sternum dislocation and wound dehiscence resulted in an infection complicating the recovery of an immunosuppressed patient after bilateral lung transplantation. Anaerobic culture (96 h) of milky cloudy wound secretion resulted in the growth of pinpoint haemolytic colonies identified as Metamycoplasma hominis (formerly Mycoplasma hominis). The search for the endogenous source of the infection found the bacterium exclusively in the patient's sputum, making a possible link to donor lung M. hominis colonization. Unfortunately, the donor samples were no longer available. The wound infection was successfully treated with 17 days of clindamycin despite the continuous PCR detection of M. hominis in the sputum after the end of the treatment.
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- COVID-19 diagnosis MeSH
- Immunocompromised Host MeSH
- Surgical Wound Infection * microbiology drug therapy diagnosis MeSH
- Clindamycin therapeutic use MeSH
- Middle Aged MeSH
- Humans MeSH
- Mycoplasma hominis * genetics isolation & purification MeSH
- Mycoplasma Infections * microbiology diagnosis drug therapy MeSH
- SARS-CoV-2 genetics isolation & purification MeSH
- Sputum microbiology MeSH
- Lung Transplantation * adverse effects MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- MeSH
- Urinalysis classification methods MeSH
- Blood Chemical Analysis classification MeSH
- Feces microbiology MeSH
- Hematologic Tests classification MeSH
- Humans MeSH
- Specimen Handling * classification methods instrumentation MeSH
- Blood Specimen Collection classification methods instrumentation MeSH
- Sputum MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
Introduction. Cystic fibrosis (CF) is a serious disease with multisystemic clinical signs that is easily and frequently complicated by bacterial infection. Recently, the prevalence of nontuberculous mycobacteria as secondary contaminants of CF has increased, with the Mycobacterium avium complex (MAC) and Mycobacterium abscessus complex (MABSC) being the most frequently identified. The MABSC includes subspecies of significant clinical importance, mainly due to their resistance to antibiotics.Gap statement. Sensitive method for early detection and differentiation of MABSC members and MAC complex for use in routine clinical laboratories is lacking. A method based on direct DNA isolation from sputum, using standard equipment in clinical laboratories and allowing uncovering of possible sample inhibition (false negative results) would be required. The availability of such a method would allow accurate and accelerated time detection of MABSC members and their timely and targeted treatment.Aim. To develop a real time multiplex assay for rapid and sensitive identification and discrimination of MABSC members and MAC complex.Methodology. The method of DNA isolation directly from the sputum of patients followed by quadruplex real-time quantitative PCR (qPCR) detection was developed and optimised. The sensitivity and limit of detection (LOD) of the qPCR was determined using human sputum samples artificially spiked with a known amount of M. abscessus subsp. massiliense (MAM).Results. The method can distinguish between MAC and MABSC members and, at the same time, to differentiate between M. abscessus subsp. abscessus/subsp. bolletii (MAAb/MAB) and MAM. The system was verified using 61 culture isolates and sputum samples from CF and non-CF patients showing 29.5 % MAAb/MAB, 14.7 % MAM and 26.2 % MAC. The LOD was determined to be 1 490 MAM cells in the sputum sample with the efficiency of DNA isolation being 95.4 %. Verification of the qPCR results with sequencing showed 100 % homology.Conclusions. The developed quadruplex qPCR assay, which is preceded by DNA extraction directly from patients' sputum without the need for culturing, significantly improves and speeds up the entire process of diagnosing CF patients and is therefore particularly suitable for use in routine laboratories.
- MeSH
- Mycobacterium Infections, Nontuberculous * diagnosis drug therapy MeSH
- Cystic Fibrosis * complications microbiology MeSH
- DNA therapeutic use MeSH
- Mycobacterium avium-intracellulare Infection * epidemiology MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Humans MeSH
- Mycobacterium abscessus * genetics MeSH
- Mycobacterium avium Complex genetics MeSH
- Nontuberculous Mycobacteria MeSH
- Sputum microbiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Keywords
- poklepové hranice, transparence plic, difuzní zastínění,
- MeSH
- Blood Gas Analysis methods MeSH
- Medical History Taking methods MeSH
- Bronchoscopy methods instrumentation MeSH
- Diagnostic Techniques, Respiratory System * classification MeSH
- Respiratory System pathology MeSH
- Physical Examination classification methods MeSH
- Thorax diagnostic imaging pathology MeSH
- Virus Cultivation MeSH
- Humans MeSH
- Specimen Handling classification methods MeSH
- Palpation classification methods MeSH
- Percussion classification MeSH
- Pleural Effusion diagnosis MeSH
- Lung diagnostic imaging pathology MeSH
- Respiration Disorders diagnosis classification MeSH
- Auscultation MeSH
- Radiography, Thoracic methods MeSH
- Respiratory Sounds classification physiopathology MeSH
- Spirometry MeSH
- Sputum MeSH
- Thoracentesis methods nursing MeSH
- Tuberculin Test methods MeSH
- Vital Capacity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Keywords
- periferní krev,
- MeSH
- Asthma physiopathology MeSH
- Eosinophils * physiology MeSH
- Humans MeSH
- Sputum MeSH
- Check Tag
- Humans MeSH
- Keywords
- deeskalace, bradypsychie,
- MeSH
- Anti-Bacterial Agents administration & dosage pharmacology classification MeSH
- Antibiotic Prophylaxis MeSH
- Antifungal Agents therapeutic use MeSH
- Bacterial Infections * diagnosis MeSH
- Clostridioides difficile pathogenicity MeSH
- Diagnosis, Differential MeSH
- Dyspnea etiology drug therapy therapy MeSH
- Endocarditis diagnosis etiology pathology MeSH
- Hypertension MeSH
- Hypotension drug therapy therapy MeSH
- Urinary Tract Infections diagnosis etiology drug therapy MeSH
- Cross Infection diagnosis etiology drug therapy classification therapy MeSH
- Community-Acquired Infections diagnosis etiology drug therapy classification therapy MeSH
- Invasive Fungal Infections diagnosis etiology drug therapy classification MeSH
- Catheter-Related Infections diagnosis therapy MeSH
- Clostridium Infections diagnosis therapy MeSH
- Colectomy MeSH
- Humans MeSH
- Meningitis, Bacterial diagnosis etiology drug therapy cerebrospinal fluid MeSH
- Meningococcal Infections diagnosis therapy MeSH
- Healthcare-Associated Pneumonia diagnosis etiology drug therapy therapy MeSH
- Oxygen Inhalation Therapy MeSH
- Pneumonia diagnosis etiology drug therapy microbiology therapy MeSH
- Disease Progression MeSH
- Diarrhea surgery etiology MeSH
- Respiratory Insufficiency MeSH
- Risk Factors MeSH
- Aged MeSH
- Sepsis etiology classification MeSH
- Sputum microbiology MeSH
- Streptococcus pneumoniae pathogenicity MeSH
- Terminology as Topic MeSH
- Check Tag
- Humans MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- Review MeSH
About one third of the population is infected with tuberculosis (TB). On the other hand, iron deficiency is the most common micronutrient deficiency in the world. A number of studies have documented anemia in patients with TB, however, this study aimed to assess the prevalence of iron deficiency anemia (IDA) in patients with acid-fast bacilli (AFB) sputum smear-positive, and sputum conversion in these two groups of patients with absolute and functional IDA at the end of the second month of anti-TB therapy in Zahedan, Iran. The results of this study revealed that 91 out of 198 (45.9%) sputum positive pulmonary TB patients were anemic, and among those 72 (79.1%) had iron deficiency anemia. The overall prevalence of IDA in this study was 36.3%. In 72 patients with IDA, 54 (75%) had functional while the remainder had absolute IDA 18 (25%). Twenty-one out of 72 (29.2%) of patients with IDA remained sputum positive and among 126 non IDA patients 47 (37.3%) had positive sputum smear at the end of intensive TB treatment phase (p=0.278). Approximately, less than half of patients with tuberculosis had anemia among them 79% had iron deficiency anemia. The frequency of functional IDA was three times more than absolute IDA. There was no statistically significant difference in sputum conversion between two groups of IDA and non-IDA patients after intensive phase of anti-TB therapy.
- MeSH
- Anemia, Iron-Deficiency * complications epidemiology MeSH
- Antitubercular Agents MeSH
- Humans MeSH
- Mycobacterium tuberculosis * MeSH
- Tuberculosis, Pulmonary * complications epidemiology MeSH
- Prevalence MeSH
- Sputum MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Iran MeSH
Asthma is a complex disease with a variable course. Efforts to identify biomarkers to predict asthma severity, the course of disease and response to treatment have not been very successful so far. Biomarker research has expanded greatly with the advancement of molecular research techniques. An ideal biomarker should be suitable to identify the disease as well the specific endotype/phenotype, useful in the monitoring of the disease and to determine the prognosis, easily to obtain with minimum discomfort or risk to the patient. An ideal biomarker should be suitable to identify the disease as well the specific endotype/phenotype, useful in the monitoring of the disease and to determine the prognosis, easily to obtain with minimum discomfort or risk to the patient - exhaled breath analysis, blood cells and serum biomarkers, sputum cells and mediators and urine metabolites could be potential biomarkers of asthma bronchiale. Unfortunately, at the moment, an ideal biomarker doesn't exist and the overlap between the biomarkers is a reality. Using panels of biomarkers could improve probably the identification of asthma endotypes in the era of precision medicine.
- MeSH
- Biomarkers blood metabolism urine MeSH
- Asthma diagnosis metabolism therapy MeSH
- Precision Medicine methods trends MeSH
- Humans MeSH
- Predictive Value of Tests MeSH
- Sputum chemistry metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Keywords
- periostin,
- MeSH
- Biomarkers * analysis MeSH
- Asthma * diagnosis therapy MeSH
- Breath Tests MeSH
- Dipeptidyl Peptidase 4 analysis MeSH
- Eosinophils chemistry MeSH
- Immunoglobulin E analysis MeSH
- Humans MeSH
- Nitric Oxide analysis MeSH
- Sputum chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
